PI3K-AKT-Bcl-2抗凋亡途径与瘢痕癌癌细胞凋亡相关性探讨

发布时间：2018-02-11 20:23

本文关键词： 瘢痕癌 PIK/AKT信号传导通路 Bcl- 凋亡　出处：《中华肿瘤防治杂志》2014年08期 　论文类型：期刊论文

【摘要】：目的:探讨PI3K/AKT信号传导通路中PI3K、AKT及其下游靶基因编码的Bcl-2蛋白与瘢痕癌癌细胞凋亡的相关性。方法:以15例病理性皮肤瘢痕被覆上皮和20例皮肤瘢痕癌组织为研究对象,以10例正常皮肤表皮组织为对照。这些标本均为石蜡包埋组织,源于遵义医学院附属第一和第五医院病理科2006-01-01-2011-12-30的外检标本。1)采用免疫组织化学技术(SP法)分别检测以上3组组织中PI3K、AKT和Bcl-2蛋白的表达。2)采用核酸分子原位杂交技术检测PI3K mRNA、AKT mRNA的表达。3)以原位末端标记法(Tunel法)检测细胞的凋亡水平。4)免疫组化和原位杂交图像运用图像分析软件计算其平均光密度和阳性面积,Tunel图像计算其凋亡指数,所得数据运用SPSS 16.0软件包进行统计学分析。结果:1)PI3K蛋白和PI3K mRNA在正常皮肤表皮、病理性瘢痕上皮和瘢痕癌组织中分别呈阴性、弱阳性和阳性表达。瘢痕癌组PI3K表达水平为0.172±0.039,表达强度为0.301±0.045,与正常皮肤组0.004±0.003和0.185±0.021,病理性瘢痕组0.011±0.009和0.203±0.034比较,差异有统计学意义,P0.01;瘢痕癌组PI3KmRNA表达水平为0.137±0.037,表达强度为0.379±0.054,与正常皮肤组0.008±0.007和0.265±0.016,病理性瘢痕组0.027±0.012和0.293±0.031比较,差异有统计学意义,P0.01;但正常皮肤组与病理性瘢痕组比较,差异无统计学意义,P0.05。2)AKT蛋白在瘢痕癌组表达水平为0.168±0.052,表达强度为0.388±0.081,与正常皮肤组0.005±0.005和0.218±0.036,病理性瘢痕组0.028±0.025和0.282±0.049比较,差异有统计学意义,P0.01;AKT mRNA在瘢痕癌组表达水平为0.144±0.032,表达强度为0.345±0.031,与正常皮肤组0.017±0.004和0.244±0.025,病理性瘢痕组0.037±0.019和0.257±0.027比较,差异有统计学意义,P0.01;正常皮肤组与病理性瘢痕组比较,差异无统计学意义,P0.05。3)Bcl-2在3组组织中均呈阴性表达。其表达水平、表达强度在正常皮肤组(0.006±0.003和0.168±0.019)、病理性瘢痕组(0.008±0.005和0.175±0.027)和瘢痕癌组(0和0.167±0.015)之间比较,差异无统计学意义,P=0.136。4)正常皮肤组和病理性瘢痕组AI分别为(8.48±0.78)%和(8.14±0.53)%,高于瘢痕癌组(6.41±0.60)%,且差异有统计学意义,P=0.01;但正常皮肤组与病理性皮肤瘢痕组比较,差异无统计学意义,P=0.129。结论:PI3K/AKT信号传导通路的抗凋亡途径,可能是通过Bcl-2以外的其他效应分子来实现的。
[Abstract]:Objective: to investigate the relationship between the apoptosis of scar cancer cells and the Bcl-2 protein encoded by PI3KnakT and its downstream gene in PI3K/AKT signal transduction pathway. Methods: 15 cases of pathological skin scar coated epithelium and 20 cases of skin scar carcinoma tissue were studied. Ten normal skin epidermis tissues were used as controls. Samples from the Department of Pathology of the first affiliated Hospital of Zunyi Medical College and the Department of Pathology of 5th Hospital, from January to 30, 2006 01-01-2011-12-30. (1) Immunohistochemical technique was used to detect the expression of PI3KFAKT and Bcl-2 protein in the tissues of the above three groups. 2) Nucleic acid in situ hybridization was used. The expression of PI3K mRNA-AKT mRNA was detected by using in situ end labeling (Tunel method). (4) Immunohistochemistry and in situ hybridization images were used to calculate the average optical density and the positive area of apoptotic index. The data were analyzed by SPSS 16.0 software package. Results the protein of PI3K and PI3K mRNA were negative in normal skin epidermis, pathological scar epithelium and scar carcinoma, respectively. The expression level of PI3K was 0.172 卤0.039 and 0.301 卤0.045 in scar carcinoma group, which was compared with that in normal skin group (0.004 卤0.003) and 0.185 卤0.021, pathological scar group (0.011 卤0.009) and pathological scar group (0.203 卤0.034). The expression level of PI3KmRNA in scar cancer group was 0.137 卤0.037 and 0.379 卤0.054, which was significantly higher than that in normal skin group (0.008 卤0.007 and 0.265 卤0.016), pathological scar group (0.027 卤0.012) and pathological scar group (0.293 卤0.031), but there was significant difference between normal skin group and pathological scar group. There was no significant difference in the expression level of P0.05.2AKT protein in the scar carcinoma group (0.168 卤0.052), and the expression intensity was 0.388 卤0.081, which was significantly higher than that in the normal skin group (0.005 卤0.005) and 0.218 卤0.036 (P < 0.05), and in the pathological scar group (0.028 卤0.025) and 0.282 卤0.049 (P < 0.05). The expression level of P0.01AKT mRNA was 0.144 卤0.032 and 0.345 卤0.031 in scar carcinoma group, which was significantly higher than that in normal skin group (0.017 卤0.004) and pathological scar group (0.257 卤0.027). There was no significant difference in the expression of P0.05.3Bcl 2 in the three groups, and the expression level of P0.05.3in normal skin group was 0.006 卤0.003 and 0.168 卤0.019 in normal skin group, 0.008 卤0.005 and 0.175 卤0.027 in pathological scar group, and 0 and 0.167 卤0.015 in scar carcinoma group. The AI of normal skin group and pathological scar group were 8.48 卤0.78% and 8.14 卤0.53, respectively, which were higher than that of scar carcinoma group (6.41 卤0.60), and the difference was statistically significant (P < 0.01), but the AI of normal skin group and pathological scar group was higher than that of pathological skin scar group. Conclusion the anti-apoptotic pathway of the 1: PI3K / AKT signaling pathway may be achieved by other effector molecules other than Bcl-2.
【作者单位】：遵义医学院珠海校区病理学教研室;遵义医学院珠海校区临床医疗系;遵义医学院附属第五医院病理科;遵义医学院附属第一医院病理科;
【基金】：贵州省科技攻关项目(2010-3080)
【分类号】：R739.5

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