IL-17和IL-23在湿疹患者中的表达及其临床意义

发布时间：2018-05-08 23:31

  本文选题：湿疹 + 白介素17　； 参考：《天津医科大学》2011年硕士论文

【摘要】：目的：湿疹是一种是由多种内外因素引起的变态反应性疾病,发病机制复杂,原因不明,极度瘙痒且易反复发作。以往认为在湿疹的发病中Th2占优势的Th1/Th2细胞亚群的失衡是其发病的一个主要机制,但此失衡理论并不能完全解释其发病机制。新近研究发现,机体存在一种新型的、以分泌IL-17为主要特征的细胞群,其在分化、生物学作用等方面不同于1型和2型T细胞,被命名为Th17细胞。IL-23也是近年新发现的细胞因子,与IL-12共同分享IL-12p40和IL-12Rβ1亚单位。研究发现,IL-23主要作用于记忆CD4+T细胞,诱导Th1型细胞的分化及IFN-γ产生,并发现其还可作用于记忆Th17细胞亚群诱导产生IL-17,对于Th17细胞的生存和活性有重要的辅助作用。因此Th17细胞亚群和IL-17、IL-23的发现为人们重新认识免疫应答和调节机制提供了方向。本实验主要研究IL-17和IL-23在湿疹患者和正正常人外周血中的表达及其差异,初步探讨1L-17和IL-23在湿疹发病机制中的作用和临床意义。 方法：参照赵辩主编的临床皮肤病学诊断标准和相关文献选取45例湿疹患者,男23例,女22例,年龄16—78岁,平均(35.68±10.58)岁。按照临床表现将患者进行分期,其中急性期患者15例,亚急性期15例,慢性期15例。以性别和年龄相匹配的30例健康体检者(男14例,女16例,年龄18—76岁,平均37.22±8.56岁)作为对照组。采用ELISA方法检测血清中IL-17和IL-23的表达水平,并收集整理湿疹患者的临床资料及实验室数据。 结果： 1.急性期和亚急性期湿疹患者血清中IL-17水平明显高于慢性期患者和正正常组(p0.05),但IL-17水平在急性期和亚急性期、慢性期和正正常组间则无统计学差异。 2.急性期和亚急性期湿疹患者血清中IL-23水平明显高于慢性期患者和正正常组(p0.05),但IL-23水平在急性期和亚急性期、慢性期和正正常组间则无统计学差异。 3.湿疹患者血清中1L-17与IL-23表达水平呈正直线相关(r=0.53,P0.05)关系。 4.湿疹患者血清中IL-17和IL-23水平与年龄、血沉、白蛋白、球蛋白、血肌酐、淋巴细胞、嗜酸性粒细胞计数和血小板计数之间无明显相关关系。 结论：IL-17和IL-23在湿疹患者血清中表达水平增高,且急性期和亚急性期更为明显,具有统计学差异。提示IL-17和IL-23可能参与了湿疹疾病的病理过程,并且与患者疾病的严重程度有关系。
[Abstract]:Objective: eczema is an allergic disease caused by a variety of internal and external factors. The pathogenesis is complex, unexplained, extremely itchy and prone to relapse. It was considered that the imbalance of the dominant Th1/Th2 cell subgroup of Th2 in the onset of eczema is a major mechanism of its pathogenesis, but this imbalance theory does not fully explain its hair. Recent studies have found that the body has a new type of cell group that secretes IL-17 as the main characteristic, which is different from 1 and 2 T cells in the differentiation and biological function, and is named Th17 cell.IL-23 as a newly discovered cytokine in recent years, sharing IL-12p40 and IL-12R beta 1 subunits together with IL-12. Research found, IL-23 It mainly acts on the memory of CD4+T cells, induces the differentiation of Th1 type cells and the production of IFN- gamma. It is found that it can also play an important role in the survival and activity of Th17 cells, which can also play an important role in the survival and activity of Th17 cells. Therefore, the discovery of Th17 cell subsets and IL-17 and the discovery of IL-23 for people to reunderstand the immune response and regulation mechanism This experiment mainly studies the expression and difference of IL-17 and IL-23 in eczema patients and normal human peripheral blood, and preliminarily discusses the role and clinical significance of 1L-17 and IL-23 in the pathogenesis of eczema.
Methods: according to the clinical dermatology diagnostic criteria and related literature, 45 cases of eczema, 23 male and 22 female, with an average age of 16 to 78 years, average (35.68 + 10.58) years, were divided according to clinical manifestations, including 15 in acute stage, 15 in subacute stage, 15 in chronic period, and 30 health with sex and age. The patients (14 men, 16 women, 18 to 76 years old and 37.22 + 8.56 years old) were used as the control group. The expression of IL-17 and IL-23 in serum was detected by ELISA method, and the clinical data and laboratory data of eczema patients were collected and collected.
Result锛，

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