女性尖锐湿疣患者人乳头瘤病毒感染情况及预后研究

发布时间：2018-05-24 17:37

本文选题：尖锐湿疣 + 人乳头瘤病毒　；参考：《中国病原生物学杂志》2017年07期

【摘要】：目的分析女性尖锐湿疣(CA)患者人乳头瘤病毒(HPV)的感染情况及基因分型,探讨HPV基因型与CA预后的关系。方法临床确诊女性CA初发患者(CA组)230例,采用导流杂交基因芯片技术(HybriMax)进行HPVDNA及基因分型检测。HPV-DNA阳性患者根据HPV基因型分为高危型、低危型和混合型3组,另选健康女性作为对照,分析HPV-DNA阳性CA患者治疗前后外周血T淋巴细胞亚群变化并与健康人进行比较;观察不同HPV基因型CA患者治疗后1、3、6个月的复发情况。结果 CA患者HPV-DNA阳性率为95.22%(219/230)。HPV-DNA阳性标本中共检出21种HPV基因型,其中HPV16(7.83%)和HPV6(29.57%)分别在高危型和低危型HPV中阳性率高。治疗前不同HPV基因型CA患者的CD_3~+、CD_4~+T淋巴细胞百分比及CD_4~+/CD8+比值均显著低于健康对照组(P0.05),CD8+T淋巴细胞百分比显著高于健康对照组(P0.05);治疗后不同HPV基因型CA患者的CD_3~+、CD_4~+T淋巴细胞百分比及CD_4~+/CD8+比值均较治疗前显著升高(P0.05),CD8+T淋巴细胞百分比显著降低(P0.05);治疗前后各HPV基因型组间T淋巴细胞亚群百分率差异无统计学意义(P0.05)。高危型患者治疗后3个月和6个月复发率分别为12.17%和15.65%,与低危型组的4.78%、6.52%和混合型组的5.56%、8.26%比较差异有统计学意义(P0.05)。结论女性CA患者HPV感染以HPV6、HPV11及HPV16 3种基因型为主,高危型和低危型HPV可单一感染也可混合感染。CA患者细胞免疫功能失调与HPV基因分型无关,但高危型HPV感染患者治疗后复发率更高。
[Abstract]:Objective to analyze the infection and genotyping of human papillomavirus (HPV) in female patients with condyloma acuminatum (CA), and to explore the relationship between HPV genotype and prognosis of CA. Methods two hundred and thirty female patients with primary CA were divided into high risk group, low risk group and mixed type group according to their HPV genotypes. The changes of T lymphocyte subsets in peripheral blood of patients with HPV-DNA positive CA before and after treatment were analyzed and compared with those of healthy subjects. The recurrence of CA patients with different HPV genotypes was observed 1 and 6 months after treatment. Results the positive rate of HPV-DNA in CA patients was 21 HPV genotypes detected in 95.22%(219/230).HPV-DNA positive specimens. HPV167.83) and HPV6 + 29.57) were detected in high risk type and low risk type HPV, respectively. The percentage of CD3 ~ T lymphocytes and the ratio of CD4 ~ / CD8 to CD4 / CD8 in CA patients with different HPV genotypes before treatment were significantly lower than those in healthy controls, and the percentage of CD3 ~ T lymphocytes in CA patients with different HPV genotypes after treatment was significantly higher than that in healthy controls. The percentage of CD3 ~ T lymphocytes and the ratio of CD4 ~ / CD8 were significantly higher than those before treatment, but the percentage of T lymphocyte subsets in all HPV genotypes before and after treatment was significantly lower than that before treatment (P0.05), and there was no significant difference in the percentage of T lymphocyte subsets between different HPV genotypes before and after treatment. The recurrence rates of high risk patients at 3 and 6 months after treatment were 12.17% and 15.65%, respectively, which were significantly different from those of low risk group (4.78%, 6.52%) and mixed group (5.56%, 8.26%). Conclusion HPV6, HPV11 and HPV16 are the main genotypes of HPV infection in female patients with CA. High risk type and low risk type HPV can be single infection or mixed infection. There is no relationship between cellular immune dysfunction and HPV genotyping in CA patients. However, the recurrence rate of high-risk HPV infection patients was higher after treatment.
【作者单位】：华中科技大学同济医学院附属武汉中心医院皮肤科;
【分类号】：R440;R752.53

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