龟头包皮炎患者淋病奈瑟菌分离与鉴定及其耐药机制研究

发布时间：2018-06-01 16:55

本文选题：龟头包皮炎 + 淋病奈瑟菌　；参考：《中国人兽共患病学报》2017年05期

【摘要】：目的分离鉴定龟头化脓性包皮炎病原菌,检测分离菌株耐药性并了解其耐药机制。方法采取尿道分泌物及龟头脓包液标本,革兰染色镜检并采用Mycoplasma IST 2试剂盒检测支原体。上述标本接种哥伦比亚血平板、淋病奈瑟菌选择平板、酵母菌鉴定平板进行细菌分离培养,获得的菌落用VITEK 2-compact全自动细菌检测分析系统进行鉴定,另采用PCR检测上述标本及菌落的淋病奈瑟菌16SrRNA基因。采用K-B法检测分离菌株对5种常用抗生素的敏感性,采用β-内酰胺酶和超广谱β-内酰胺酶确证试验了解该菌株产酶情况,PCR检测该菌株耐药相关tetM、TEM、mefA和ermF基因。结果各标本支原体检测结果均为阴性。尿道分泌物标本分离培养结果均为阴性,龟头脓包液血平板和选择平板培养结果为阳性。VITEK 2-compact系统和16SrRNA-PCR检测结果显示分离菌株为淋病奈瑟菌。该菌株产β内酰胺酶且对青霉素G、环丙沙星、四环素耐药,其基因组携带tetM、TEM、mefA和ermF基因。结论淋病奈瑟菌可引起龟头化脓性包皮炎,该淋病奈瑟菌菌株多重耐药并与其携带的耐药基因密切相关。
[Abstract]:Objective to isolate and identify the pathogen of suppurative foreskin inflammation of glans, to detect the drug resistance of the isolates and to understand the mechanism of drug resistance. Methods the specimens of urethral secretions and glans' pyogenic fluid were examined by Gram staining and Mycoplasma IST 2 kit was used to detect mycoplasma. The above specimens were inoculated with Colombian blood plate, Neisseria gonorrhoeae plate was selected, yeast identification plate was used to isolate and culture bacteria, and the colony was identified by VITEK 2-compact automatic bacterial detection and analysis system. The 16SrRNA gene of Neisseria gonorrhoeae was detected by PCR. K-B method was used to detect the sensitivity of the isolated strains to five commonly used antibiotics. 尾 -lactamases and extended-spectrum 尾 -lactamases were used to confirm the enzyme production of the strain. PCR was used to detect the resistance related tetMTEMmefA and ermF genes. Results the results of mycoplasma were negative. The isolated and cultured specimens of urethral secretions were all negative. The results of blood plate culture and selected plate culture were positive. VITEK 2-compact system and 16SrRNA-PCR analysis showed that the isolated strains were Neisseria gonorrhoeae (Neisseria gonorrhoeae). The strain produced 尾 -lactamases and was resistant to penicillin G, ciprofloxacin and tetracycline, and its genome carried the genes of tetMtMfA and ermF. Conclusion Neisseria gonorrhoeae can cause suppurative preputial inflammation of glans. The strain of Neisseria gonorrhoeae is multidrug resistant and closely related to the drug resistance genes it carries.
【作者单位】：浙江省人民医院检验中心;
【基金】：浙江省医药卫生科技计划项目(2017KY004)资助~~
【分类号】：R446.5;R759.2

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