雌、孕激素对淋球菌诱导的Hela细胞TSLP和TGF-β表达的影响

发布时间：2018-07-08 10:18

本文选题：淋球菌 + 雌激素　；参考：《中国皮肤性病学杂志》2014年05期

【摘要】：目的探讨雌、孕激素对淋球菌(Ng)诱导的人宫颈癌细胞胸腺基质淋巴细胞生成素(TSLP)和转化生长因子β(TGF-β)表达的影响。方法体外培养宫颈癌细胞株Hela,将获得的Hela细胞分为如下5组:对照组、Ng组、Ng+雌激素组、Ng+孕激素组、Ng+雌激素+孕激素组。分别培养24h后应用实时荧光定量PCR检测各组细胞TSLP和TGF-βmRNA表达变化情况,Western blot检测各组细胞TSLP和TGF-β蛋白水平。结果 Hela细胞在淋球菌的刺激下,细胞TSLP和TGF-βmRNA及蛋白表达水平增高(P㩳0.01);雌、孕激素单独或联合作用24h后都可以显著提高Hela细胞中TSLP和TGF-βmRNA表达水平(P㩳0.01)。结论 Hela细胞在淋球菌作用下合成TSLP和TGF-β增加;提示雌、孕可促进淋球菌诱导的TSLP和TGF-β合成。
[Abstract]:Objective to investigate the effects of estrogen and progesterone on the expression of thymus stromal lymphopoietin (TSLP) and transforming growth factor 尾 (TGF- 尾) induced by gonorrhoeae (Ng) in human cervical cancer cells. Methods the cervical cancer cell line Hela was cultured in vitro. The Hela cells were divided into 5 groups: control group Ng estrogen group Ng progesterone group Ng estrogen progesterone group. The changes of TSLP and TGF- 尾 mRNA expression in each group were detected by real-time fluorescence quantitative PCR after 24 h culture respectively. Western blot was used to detect the TSLP and TGF- 尾 protein levels in each group. Results the expression of TSLP and TGF- 尾 mRNA and protein were increased in Hela cells stimulated by Neisseria gonorrhoeae (P0. 01), and the expression levels of TSLP and TGF- 尾 mRNA in Hela cells were significantly increased by estrogen and progesterone alone or in combination for 24 h (Pf0.01). Conclusion the synthesis of TSLP and TGF- 尾 in Hela cells under the action of Neisseria gonorrhoeae is increased, suggesting that pregnancy can promote the synthesis of TSLP and TGF- 尾 induced by Neisseria gonorrhoeae.
【作者单位】：武汉市第一医院皮肤科;华中科技大学同济医学院附属协和医院皮肤科;
【基金】：国家自然科学基金项目(81101201,81171495,81271765)
【分类号】：R759.2;R737.33

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