复活草提取物调控雪旺细胞损伤后水通道蛋白1的表达作用的研究

发布时间：2018-02-01 02:24

本文关键词： 复活草提取物周围神经雪旺细胞水通道蛋白　出处：《临床耳鼻咽喉头颈外科杂志》2017年07期 　论文类型：期刊论文

【摘要】：目的:探讨缺氧诱导雪旺细胞膜水通道蛋白1(AQP1)表达机制及复活草提取物对缺氧损伤细胞的修复作用。方法:以不同浓度的CoCl_2(2、4、6、8、10μl/ml)培养细胞贴壁24h后,显微镜下观察细胞形态变化拍照并以Western Blot检测AQP1变化,确定最适浓度的CoCl_2进行后续试验。以不同浓度的复活草提取物(0.5、1.0、2.5、5.0、10.0μl/ml)培养贴壁细胞,24h后进行CCK-8检测。选取最适浓度的CoCl_2及复活草提取物进行后续试验,分为空白对照组、缺氧模型组、复活草提取物修复组观察7d,Western Blot检测7d的细胞AQP1及HIF-1α变化。结果:选取6μl/ml CoCl_2为最适浓度缺氧模型,细胞形态变化明显但对细胞生长未见明显抑制作用,AQP1表达含量最高。选取2.5μl/ml复活草提取物为最适浓度,能够明显促进细胞增殖。HIF-1α诱导雪旺细胞AQP1表达增加,复活草提取物能下调AQP1及HIF-1α的表达。结论:周围神经水肿与雪旺细胞AQP1的表达有明显相关性,细胞缺氧后HIF-1α诱导AQP1表达增加;复活草提取物能够明显下调缺氧的雪旺细胞中AQP1的变化从而减轻水肿。
[Abstract]:Objective: to investigate the mechanism of Hypoxia-induced aquaporin-1aquaporin-1 (AQP1) expression in Schwann cell membrane and the repair effect of the extract of Rhizoma Rehmannifera on hypoxic injury cells. Cell morphology was observed under microscope and AQP1 was detected by Western Blot. The optimal concentration of CoCl_2 was determined for the follow-up test. Adherent cells were cultured with different concentrations of Rhizoma Rehmanniae extract (0.5lml) and 2.50.5.0g / ml (10.0 渭 l / ml). After 24 hours, CCK-8 was detected. The optimal concentration of CoCl_2 and the extract of Rhizoma reviviflora were selected for follow-up test and divided into three groups: blank control group, hypoxia model group and rebirth grass extract repair group for 7 days. Western Blot was used to detect the changes of AQP1 and HIF-1 伪 in cells for 7 days. Results: 6 渭 l / ml CoCl_2 was selected as the optimal hypoxia model. The expression of AQP1 was the highest in the cells with obvious morphological changes but no obvious inhibition on cell growth. The optimal concentration was 2.5 渭 l / ml. HIF-1 伪 can obviously promote cell proliferation. HIF-1 伪 induces the increase of AQP1 expression in Schwann cells. Conclusion: the expression of AQP1 in Schwann cells is significantly correlated with peripheral nerve edema. The expression of AQP1 was increased by HIF-1 伪 after hypoxia. The extract of Rhizoma Resustifolia could significantly downregulate the changes of AQP1 in the hypoxic Schwann cells and alleviate the edema.
【作者单位】：天津医科大学总医院耳鼻咽喉科;天津医科大学总医院皮肤与性病科;
【分类号】：R745.12
【正文快照】： 1天津医科大学总医院耳鼻咽喉科(天津,300000)2天津医科大学总医院皮肤与性病科随着社会的发展,事故外伤、手术等引起的周围神经损伤逐渐增多,以面神经损伤引起的面瘫在耳鼻咽喉科尤为常见,由于其特殊的解剖结构,周围性面瘫发病率高并且治疗效果不佳。面神经走形于狭窄的骨性

【相似文献】