微小RNA-181b通过调节热休克蛋白A5在缺血性脑卒中小鼠中的作用

发布时间：2018-03-26 01:17

本文选题：大脑中动脉阻塞　切入点：微小RNA-b　出处：《解剖学报》2017年01期

【摘要】：目的探讨微小RNA-181b(miR-181b)在缺血性脑卒中小鼠脑损伤中的作用及机制。方法采用小鼠大脑中动脉阻塞(MCAO)模型模拟缺血性脑损伤;Real-time PCR检测miR-181b mRNA表达情况;蛋白印迹观察miR-181b靶蛋白热休克蛋白A5(HSP A5)蛋白水平变化情况;Nissl方法检测皮层缺血神经元的缺失情况;行为学方法评估小鼠的神经行为学功能损伤程度。结果侧脑室注射miR-181b拮抗剂可使脑内miR-181b表达水平明显降低(P0.05,n=3);MCAO后小鼠的神经行为学功能受到严重损伤,miR-181b拮抗剂可明显缓解这些行为学改变(P0.05,n=6);下调miR-181b可以提高HSPA5蛋白水平(P0.05,n=3);MCAO后小鼠皮层有一定程度的神经细胞缺失,预先给予miR-181b拮抗剂发挥保护作用,神经细胞缺失减少(P0.05,n=3)。结论 miR-181b可能通过调节HSPA5蛋白的表达在缺血性脑卒中小鼠脑损伤中发挥重要作用。
[Abstract]:Objective to investigate the role and mechanism of minimal RNA-181bmmiR-181b in cerebral injury in mice with ischemic stroke. Methods the expression of miR-181b mRNA was detected by real-time PCR of middle cerebral artery occlusion (MCAO) model in mice. The changes of miR-181b target protein A5(HSP A5 protein levels were observed by Western blot. The loss of ischemic neurons in cortex was detected by Nissl method. Results injection of miR-181b antagonist into lateral ventricle could significantly decrease the expression of miR-181b in the brain of mice. The decrease of miR-181b could increase the level of HSPA5 protein and the loss of neurons in the cortex of mice after P0.05 nonglucifera (MCAO), and decrease the level of P0.05, P0.05, P0. 05, P0. 05, P0. 05, P0. 05, P0. 05, P0. Pretreatment with miR-181b antagonist may play a protective role, and the loss of nerve cells will be reduced by P0. 05. Conclusion miR-181b may play an important role in cerebral injury by regulating the expression of HSPA5 protein in ischemic stroke mice. [WT5 "HZ] [WT5" BZ] [WT5 "BZ] [WT5" BZ]
【作者单位】：山西大同大学医学院生理学教研室;
【基金】：山西大同大学博士科研启动经费(2014-B-01) 山西省基础研究项目(2015021178)
【分类号】：R743.3

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