C1型尼曼-匹克症小鼠脊髓胶质细胞的活性变化

发布时间：2018-04-13 19:18

本文选题：C型尼曼-匹克症 + 脊髓　；参考：《解剖学报》2017年03期

【摘要】：目的通过观察C1型尼曼-匹克症小鼠不同脊髓节段星形胶质细胞和小胶质细胞的活性变化,探讨Npc1基因突变对脊髓发育的影响。方法 Npc1~(+/-)小鼠交配繁殖产生Npc~(1-/-)(n=3)和Npc1~(+/+)小鼠(n=3),PCR检测新生小鼠的基因型;选取35日龄的Npc1~(-/-)和Npc1~(+/+)小鼠,采用免疫荧光方法观察对比脊髓不同节段(颈、胸、腰、骶)星形胶质细胞和小胶质细胞的活性变化。采用免疫双染色检测胶质细胞中炎性因子的表达情况,采用免疫印迹方法检测白细胞介素-1β(IL-1β)、SMI31和磷酸化的tau蛋白表达情况。结果在35日龄Npc1~(-/-)小鼠脊髓的各个节段,其背角和腹角的星形胶质细胞和小胶质细胞的活性均明显增强(P0.05),并伴随细胞炎性因子IL-1β表达量的显著增加;同时,脊髓神经丝蛋白和骨架蛋白tau蛋白发生超磷酸化。结论 Npc1基因突变引起脊髓神经胶质细胞发生病理性变化,可能是脊髓神经元病理性损伤的重要原因。
[Abstract]:Objective to investigate the effect of Npc1 gene mutation on spinal cord development by observing the activity changes of astrocytes and microglia in different segments of spinal cord in mice with C1 type Neiman-peak syndrome.Methods the genotypes of newborn mice were detected by PCR in Npc1 / 1 / 1 / Npc1 (/) mice and Npc1 / 1 / 1 (/) mice, and 35 days old Npc1 / P /-and Npc1 / (/) mice were used to observe and compare different segments of spinal cord (neck, chest, waist, waist, and / or Npc1) by immunofluorescence method.Sacral) changes in the activity of astrocytes and microglia.The expression of inflammatory cytokines in glial cells was detected by double immunostaining, and the expression of interleukin-1 尾-尾 -IL-1 尾 -simi31 and phosphorylated tau protein were detected by Western blotting.Results the activities of astrocytes and microglia in the dorsal horn and ventral horn of 35 day old NPC1 / r-伪 mice were significantly increased, accompanied by a significant increase in the expression of cytokine IL-1 尾.Spinal cord neurofilament protein and skeleton protein tau protein were hyperphosphorylated.Conclusion the pathological changes of spinal glial cells caused by mutation of Npc1 gene may be an important cause of pathological injury of spinal cord neurons.
【作者单位】：新乡医学院生命科学技术学院;河南省医用组织再生重点实验室;
【基金】：国家自然科学基金(81400936,U1304808,81600987) 河南省高等学校重点科研项目(16A180014) 河南省高校科技创新人才支持计划(14HASTIT032) 河南省科技发展计划(142300410192) 新乡医学院研究生科研创新支持计划(YJSCX201516Y)
【分类号】：R741

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