α-硫辛酸对脑缺血再灌注损伤大鼠氧化应激的影响

发布时间：2018-04-26 13:10

本文选题：硫辛酸 + 脑缺血　；参考：《中华老年心脑血管病杂志》2016年09期

【摘要】：目的探讨α-硫辛酸对大鼠脑缺血再灌注损伤氧化应激的影响。方法将SD大鼠96只随机分为假手术组、模型组和干预组(α-硫辛酸腹腔注射),每组32只,后2组应用线栓法制备大脑中动脉闭塞2h后再灌注模型,根据再灌注时间6、24h、3、7d4个时间点各取8只,采用TTC染色法测定脑梗死灶大小,进行神经功能缺损评分,检测脑组织中超氧化物歧化酶(SOD)、丙二醛、NO含量及诱导型一氧化氮合酶(iNOS)活性。结果与模型组比较,干预组6、24h、3、7d脑组织中的丙二醛、NO及iNOS活性显著降低(P0.05),SOD活性显著升高[(115.46±1.14)mU/L vs(103.44±1.16)mU/L,(128.88±1.16)mU/L vs(109.12±1.12)mU/L,(120.26±1.14)mU/L vs(107.26±1.10)mU/L,(116.56±1.24)mU/L vs(104.82±1.21)mU/L,P0.05]。结论α-硫辛酸能改善大鼠脑缺血再灌注损伤,其机制可能是α-硫辛酸清除自由基抗氧化作用。
[Abstract]:Objective to investigate the effect of 伪-lipoic acid on oxidative stress during cerebral ischemia reperfusion injury in rats. Methods Ninety-six Sprague-Dawley rats were randomly divided into sham-operated group, model group and intervention group (32 rats in each group were injected with 伪 -lipoic acid). According to the reperfusion time of 6 ~ 24 hours and 3 ~ 7 days, 8 rats were taken from each time point. The size of cerebral infarction was measured by TTC staining, the neurological impairment score was evaluated, the content of superoxide dismutase (SOD), malondialdehyde (MDA) and the activity of inducible nitric oxide synthase (iNOS) in brain tissue were detected. Results compared with the model group, the activity of malondialdehyde (no) and iNOS in the brain tissue of the intervention group decreased significantly at 624 hours and 3 days after the treatment. The activity of iNOS in the brain tissue was significantly increased [115.46 卤1.16mU / L, 128.88 卤1.12mU / L, 120.26 卤1.10mU / L, 116.56 卤1.24)mU/L vs(104.82 卤1.21mU / L, P 0.05, respectively, but also significantly higher than that in the control group (128.88 卤1.12mU / L, 128.88 卤1.12mU / L + 1.10mU / L = 116.56 卤1.21mU / L, P 0.05). Conclusion 伪 -lipoic acid can improve cerebral ischemia-reperfusion injury in rats, and its mechanism may be the scavenging effect of 伪 -lipoic acid on free radical.
【作者单位】：天津医科大学第四中心临床学院神经内科;
【分类号】：R743.3

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