石杉碱甲对帕金森病模型大鼠乙酰胆碱酯酶及ATP酶活性的影响

发布时间：2018-05-12 07:48

本文选题：帕金森病 + 乙酰胆碱酯酶　；参考：《重庆医学》2017年22期

【摘要】：目的探讨石杉碱甲对帕金森模型病大鼠乙酰胆碱酯酶(AchE)及ATP酶活性的影响。方法选择45只雄性SD大鼠为研究对象,采用随机数字表法分为空白对照组、模型组、石杉碱甲组各15只。模型组、石杉碱甲组采用6-羟基多巴胺脑立体定向单侧纹状体双靶点微量注射法制备帕金森大鼠模型,石杉碱甲组给予0.1mg·kg-1·d-1石杉碱甲灌胃,空白对照组和模型组灌入等容量蒸馏水。比较3组大鼠空间记忆能力、纹状体多巴胺(DA)水平及AchE水平、脑内黑质Ca~(2+)-ATPase、Na~+-K~+-ATPase、Ca~(2+)-Mg~(2+)-ATPase活性。结果模型组信息游泳时间、选择游泳时间明显长于空白对照组(t=6.907、6.966,P0.05),石杉碱甲组大鼠信息游泳时间、选择游泳时间明显短于模型组(t=4.572、3.444,P0.05),且石杉碱甲组大鼠且明显短于模型组(t=2.421、3.912,P0.05);模型组大鼠脑组织多巴胺明显低于空白对照组,AchE明显高于空白对照组(t=12.517,6.834,P0.05),石杉碱甲组大鼠脑组织多巴胺明显高于模型组,AchE明显低于模型组(t=6.388、4.719,P0.05),且石杉碱组大鼠脑组织多巴胺明显低于空白对照组,AchE明显高于空白对照组(t=6.055、2.024,P0.05);模型组大鼠脑黑质中Ca~(2+)-ATPase、Na~+-K~+-ATPase、Ca~(2+)-Mg~(2+)-ATPase水平均明显低于空白对照组(t=20.326、15.2225、12.517,P0.05),石杉碱甲组大鼠脑黑质中Ca~(2+)-ATPase、Na~+-K~+-ATPase、Ca~(2+)-Mg~(2+)-ATPase水平均高于模型组(t=16.771、9.516、7.480,P0.05),且大鼠脑黑质中Ca~(2+)-ATPase、Na~+-K~+-ATPase、Ca~(2+)-Mg~(2+)-ATPase水平均低于模型组(t=3.901、5.844、5.848,P0.05)。结论石杉碱甲有助于提高帕金森大鼠空间记忆能力,可能与抑制AchE表达、提高脑组织中多巴胺及ATP酶的活性有关。
[Abstract]:Objective to investigate the effects of Huperzine A on the activities of acetylcholinesterase (ache) and ATP in Parkinson's disease (PD) rats. Methods Forty-five male Sprague-Dawley rats were randomly divided into control group (n = 15) and Huperzine A group (n = 15). Model group, Huperzine A group, 6-hydroxydopamine stereotactic unilateral striatal microinjection was used to make Parkinson rat model. Huperzine A group was given 0.1mg kg-1 d-1 Huperzine A intragastric perfusion. Blank control group and model group were perfused with distilled water of equal capacity. The spatial memory ability, striatal dopamine (DA) level and AchE level in the three groups were compared, and the activity of Ca~(2 ~ -K ~ -ATPasesi-Na ~ -K ~ -AT Pasea-CaO2-Mg-PS-ATPase in the substantia nigra was compared. Results the information swimming time in the model group was significantly longer than that in the blank control group. The swimming time in the model group was significantly shorter than that in the model group, and that in the Huperzine A group was significantly shorter than that in the model group, and the dopamine in the brain tissue of the model group was significantly lower than that in the blank control group, and that in the model group was significantly higher than that in the blank control group (t12.5176.834, P 0.05), and that in the Huperzine A group, the brain tissue of the model group was significantly lower than that of the blank control group, and that of the rats in the Huperzine A group was significantly higher than that in the control group. The tissue dopamine was significantly higher than that in the model group (6.3884.719 P0.05), and the dopamine in the brain tissue of the Huperzine group was significantly lower than that of the blank control group (P 0.05). The contents of Ca~(2 -ATPaseNa ~ -K ~ -ATPase2 + -ATPase water in the substantia nigra of the model group were significantly higher than that of the blank control group (6.055 ~ 2.024) P0.05; the content of Ca~(2 -ATPaseNa ~ -K ~ -ATPase2 in the substantia nigra of the model group was significantly higher than that of the blank control group (P0.05). The average level of Ca~(2 in the substantia nigra of rats in Huperzine A group was significantly lower than that in the blank control group. The levels of Ca~(2 ~ -K ~ -ATPaseCaO2 + -MgP0. 05-ATPase in the substantia nigra of rats were all higher than those in the model group (t16.7719.5167.480P0.05), and the levels of Ca~(2 -ATPaseNa ~ -K ~ -ATPase2 -Mg2 in the substantia nigra of rats were lower than those of the model group. Conclusion Huperzine A can improve the spatial memory ability of Parkinson's rats, which may be related to the inhibition of AchE expression and the increase of dopamine and ATP enzyme activities in brain tissue.
【作者单位】：广西壮族自治区人民医院神经内科干部病区老年神经内科;广西医科大学第一附属医院检验科;广西医科大学药学院;
【分类号】：R-332;R742.5

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