外源性神经节苷脂联合灵芝三萜化合物对癫痫脑损伤神经保护作用

发布时间：2018-06-29 18:30

本文选题：癫痫 + GM　；参考：《现代预防医学》2017年04期

【摘要】：目的探讨灵芝三萜类化合物联合外源性神经节苷脂(GM1)对癫痫脑损伤早期神经保护作用的效果及机制。方法Sprague-Dawley大鼠随机分为空白对照组、癫痫模型组、灵芝三萜化合物组、溶媒对照组、GM1组、GM1联合灵芝三萜化合物组,除空白对照组外其余各组用戊四氮亚惊厥剂量腹腔注射,复制慢性癫痫模型。双抗体夹心ELISA检测血清和脑脊液中S蛋白-β(S-100β)、C反应蛋白(CRP)及降钙素基因相关肽(CGRP)蛋白含量,放射免疫法检测神经元特异性烯醇化酶(NSE)蛋白含量。结果分别应用灵芝三萜化合物和GM1干预后,大鼠脑脊液和血清S-100β、NSE、CRP蛋白含量与癫痫模型组相比明显降低(P0.05)、CGRP含量升高(P0.05),而联合应用两种药物治疗后,大鼠癫痫发作潜伏期明显延长(P0.05),S-100β、NSE、CRP蛋白含量降低与CGRP含量升高较单一用药效果更明显(P0.05)。结论灵芝三萜化合物或GM1治疗均可不同程度减轻癫痫脑损伤,而2种药物联合应用对其脑损伤的改善作用更明显,其作用机制可能为通过影响反映脑组织损伤和炎症反应的S-100β、NSE、CRP及CGRP蛋白表达而起到脑神经元保护作用。
[Abstract]:Objective to investigate the effect and mechanism of Ganoderma lucidum triterpenoids combined with exogenous ganglioside (GM1) on early neuroprotective effect of epileptic brain injury. Methods Sprague-Dawley rats were randomly divided into three groups: control group, epileptic model group, Ganoderma lucidum triterpene compound group, GM1 group and GM1 combined with Ganoderma lucidum triterpene compound group. The other groups were injected intraperitoneally with pentylenetetrazole (PTZ) subconvulsive dose except the blank control group. The model of chronic epilepsy was established. The contents of S-100 尾 (S-100 尾) C-reactive protein (CRP) and calcitonin gene-related peptide (CGRP) in serum and cerebrospinal fluid (CSF) were detected by double antibody sandwich Elisa, and the contents of neuron-specific enolase (NSE) protein were detected by radioimmunoassay. Results after the intervention of Ganoderma lucidum triterpenoids and GM1, the contents of S-100 尾 -NSE-CRP protein in cerebrospinal fluid and serum of rats were significantly lower than those in epileptic model group (P0.05). The latency of epileptic seizure in rats was significantly prolonged (P0.05). The decrease of CRP protein content and the increase of CGRP content in S-100 尾 -NSEP were more obvious than those of single medication (P0.05). Conclusion Ganoderma lucidum triterpenoid compound or GM1 can alleviate epilepsy brain injury in different degree, and the effect of two drugs combined with Ganoderma lucidum triterpenoid compound on brain injury is more obvious. The mechanism may play a neuroprotective role by affecting the expression of S-100 尾 -NSEP CRP and CGRP protein, which reflects the injury and inflammation of brain tissue.
【作者单位】：右江民族医学院病理生理学教研室广西高校桂西地区高发病防治研究重点实验室;广西大学发展规划处;广西中医药大学;
【基金】：广西自然科学基金项目(2016GXNSFAA380278,2014GXNSFBA118172) 广西高校科学技术研究项目(KY2015YB229) 广西高校重点实验室开放课题(gxzdsysyy2015207)
【分类号】：R742.1

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