脾和腰部淋巴结切除缓解大鼠胫神经损伤后疼痛慢性化

发布时间：2018-08-29 19:43

【摘要】：目的探讨外周淋巴器官在成年大鼠胫神经永久性横断伤(即改良型备用性神经损伤,modified spared nerve injury,mSNI)后机械性疼痛异常的慢性转化中的作用。方法 30只成年雄性Sprague-Dawley(SD)大鼠随机分为3组,每组10只动物,分别用于脾脏切除、腰部淋巴结切除和右侧乆窝淋巴结-腹股沟淋巴结联合切除实验。每项实验的10只动物再随机分为外周淋巴器官切除手术组(n=5)和假手术组(n=5)。所有动物根据实验分组进行相应的外周淋巴器官切除的手术或者假手术,术后1周进行右侧mSNI手术。所有动物于外周淋巴器官切除术前、右侧mSNI手术前、mSNI手术后7d和mSNI手术后14d进行von Frey纤维试验,测试mSNI手术同侧和对侧大鼠后爪足底面腓肠神经和隐神经皮肤区域特异性的疼痛反应。结果脾脏和腰部淋巴结切除明显缓解mSNI后7d和(或)14d的大鼠手术侧后爪足底面腓肠神经和隐神经皮肤区域的机械性疼痛异常。然而,右侧胭窝淋巴结-腹股沟淋巴结联合切除对mSNI后7d和14d的大鼠手术侧后爪足底面腓肠神经和隐神经皮肤区域的机碱性疼痛异常没有显著影响。结论脾脏和腰部淋巴结参与成年大鼠mSNI后机械性疼痛异常的慢性转化。
[Abstract]:Objective to investigate the role of peripheral lymphoid organs in the chronic transformation of abnormal mechanical pain after permanent transection of tibial nerve (modified spared nerve injury,mSNI) in adult rats. Methods Thirty adult male Sprague-Dawley (SD) rats were randomly divided into 3 groups, 10 animals in each group, which were used for splenectomy, lumbar lymphadenectomy, and combined excision of right and right ventral lymph nodes and inguinal lymph nodes. Ten animals in each experiment were randomly divided into two groups: peripheral lymphotomy group (N5) and sham operation group (N5). All animals were treated with peripheral lymphotomy or sham-operation according to the experimental group, and the right mSNI was performed 1 week after operation. All animals were tested for von Frey fibers before peripheral lymphotomy, 7 days after right mSNI operation and 14 days after mSNI operation. The region specific pain response of sural nerve and saphenous nerve was measured in ipsilateral and contralateral mSNI rats. Results splenectomy and lumbar lymphadenectomy significantly alleviated the abnormal mechanical pain in the sural nerve and saphenous nerve region of the lateral sural nerve and saphenous nerve of the rats at 7 and / or 14 days after mSNI. However, the combined excision of the right popliteal and inguinal lymph nodes had no significant effect on the pathogenesis of alkaline pain in the sural nerve and saphenous nerve skin region of the lateral paw of rats 7 and 14 days after mSNI. Conclusion spleen and lumbar lymph nodes participate in the chronic transformation of abnormal mechanical pain after mSNI in adult rats.
【作者单位】： 四川大学华西基础医学与法医学院组织胚胎学与神经生物学教研室;
【基金】：国家自然科学基金面上项目(31571240)
【分类号】：R745

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