银杏叶提取物对红藻氨酸致癫痫大鼠γ-氨基丁酸A型受体及N-甲基-D-天冬氨酸受体表达的影响
发布时间：2019-01-07 19:35
【摘要】：目的观察银杏叶提取物（Ginkgo biloba extract，GBE）预处理给药和急性期给药对癫痫的干预效应及其NR2A受体及GABAA受体表达的关系，以探讨GBE的抗癫痫机制。 方法采用侧脑室注射红藻氨酸（Kainic acid, KA）建立癫痫动物模型。将108只SD大鼠随机分为假手术组（n=18）、GBE对照组(n=18)、癫痫模型组(n=18)、GBE预处理组(n=18)、GBE急性给药1组(n=18)、GBE急性给药2组(n=18)。各组再分为6h、12h及24h亚组（n=6）。应用HE染色观察海马组织中神经元的细胞形态变化，用原位杂交技术测定海马组织中NR-2AmRNA及GABA-AR mRNA的表达变化。 结果GBE预处理组和GBE急性给药1组癫痫发作级别及神经元损害较癫痫模型组降低，且以GBE预处理组损害减轻更明显。癫痫模型组3个亚组的大鼠海马组织中NR-2AmRNA表达较假手术组相对应各亚组明显增多（P0.05）；GBE预处理组及GBE急性给药1组中3个亚组大鼠海马组织中NR-2AmRNA表达明显低于癫痫模型组相对应各亚组（P0.05），GABA-ARmRNA表达较癫痫模型组相对应各亚组明显增多（P0.05）；GBE预处理组与急性期给药1组各亚组相比GABA-ARmRNA的表达上升（P0.05），，NR-2AmRNA的表达下降（P0.05）；癫痫模型组与GBE对照组各亚组相比相关指标均无统计学差异（P0.05）；急性给药模型2组3个亚组的大鼠海马组织中NR-2AmRNA的表达较急性模型1组相相对应各亚组下降。同组内6h、12h、24h三个时间点NR-2AmRNA表达呈下降趋势，而GABA-ARmRNA表达则呈上升趋势。 结论： GBE干预有一定的抗癫痫作用，且预处理给药比急性期给药效果更好；其抗癫痫的机制与减轻神经元的损害、减轻癫痫发作程度、降低NR-2AmRNA的表达及上调GABA-ARmRNA的表达有关。
[Abstract]:Objective to investigate the effects of (Ginkgo biloba extract,GBE preconditioning and acute administration on epilepsy and the expression of NR2A receptor and GABAA receptor in order to explore the antiepileptic mechanism of GBE. Methods epileptic animal model was established by intracerebroventricular injection of kainic acid (Kainic acid, KA). 108 SD rats were randomly divided into sham operation group (n = 18), GBE control group) and epileptic model group (n = 18), GBE pretreatment group). Each group was subdivided into 6 h, 12 h and 24 h subgroups (nong6). The morphological changes of neurons in hippocampus were observed by HE staining and the expression of NR-2AmRNA and GABA-AR mRNA in hippocampal tissue were detected by in situ hybridization. Results Epilepsy grade and neuronal damage in GBE preconditioning group and GBE acute administration group were lower than those in epileptic model group, especially in GBE preconditioning group. The expression of NR-2AmRNA in hippocampus of the epileptic model group was significantly higher than that of the sham operation group (P0.05). The expression of NR-2AmRNA in hippocampus of GBE preconditioning group and GBE acute administration group was significantly lower than that of epileptic model group (P0.05). The expression of GABA-ARmRNA was significantly higher than that of epileptic model group (P0.05). The expression of GABA-ARmRNA increased and the expression of NR-2AmRNA decreased in GBE preconditioning group compared with that in acute phase group 1 (P0.05). There was no significant difference in the relative indexes between the epileptic model group and the GBE control group (P0.05), and the expression of NR-2AmRNA in hippocampus of the acute model group 2 was lower than that of the acute model group 1. In the same group, the expression of NR-2AmRNA decreased at 6 h, 12 h and 24 h, while the expression of GABA-ARmRNA increased. Conclusion: GBE intervention has some antiepileptic effect, and pretreatment is better than acute administration. Its antiepileptic mechanism is related to the reduction of neuron damage, the reduction of epileptic seizures, the reduction of NR-2AmRNA expression and the up-regulation of GABA-ARmRNA expression.
【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R742.1
本文编号：2404060
[Abstract]:Objective to investigate the effects of (Ginkgo biloba extract,GBE preconditioning and acute administration on epilepsy and the expression of NR2A receptor and GABAA receptor in order to explore the antiepileptic mechanism of GBE. Methods epileptic animal model was established by intracerebroventricular injection of kainic acid (Kainic acid, KA). 108 SD rats were randomly divided into sham operation group (n = 18), GBE control group) and epileptic model group (n = 18), GBE pretreatment group). Each group was subdivided into 6 h, 12 h and 24 h subgroups (nong6). The morphological changes of neurons in hippocampus were observed by HE staining and the expression of NR-2AmRNA and GABA-AR mRNA in hippocampal tissue were detected by in situ hybridization. Results Epilepsy grade and neuronal damage in GBE preconditioning group and GBE acute administration group were lower than those in epileptic model group, especially in GBE preconditioning group. The expression of NR-2AmRNA in hippocampus of the epileptic model group was significantly higher than that of the sham operation group (P0.05). The expression of NR-2AmRNA in hippocampus of GBE preconditioning group and GBE acute administration group was significantly lower than that of epileptic model group (P0.05). The expression of GABA-ARmRNA was significantly higher than that of epileptic model group (P0.05). The expression of GABA-ARmRNA increased and the expression of NR-2AmRNA decreased in GBE preconditioning group compared with that in acute phase group 1 (P0.05). There was no significant difference in the relative indexes between the epileptic model group and the GBE control group (P0.05), and the expression of NR-2AmRNA in hippocampus of the acute model group 2 was lower than that of the acute model group 1. In the same group, the expression of NR-2AmRNA decreased at 6 h, 12 h and 24 h, while the expression of GABA-ARmRNA increased. Conclusion: GBE intervention has some antiepileptic effect, and pretreatment is better than acute administration. Its antiepileptic mechanism is related to the reduction of neuron damage, the reduction of epileptic seizures, the reduction of NR-2AmRNA expression and the up-regulation of GABA-ARmRNA expression.
【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R742.1
本文编号：2404060
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