Neurexin、Neuroligin基因在人类及鼠类动物肠神经系统的表达及意义

发布时间：2018-05-31 13:19

本文选题：Neurexin + Neuroligin基因　；参考：《山东大学》2010年硕士论文

【摘要】： 目的：探讨Neurexin和Neuroligin基因在人类及鼠类动物肠神经系统(ENS)是否有表达,以及对ENS突触结构与功能的作用,探查其在先天性巨结肠各段的表达特征及意义。方法：首先制备小鼠、大鼠、豚鼠、正常人体肠管肌间神经丛铺片,通过双重免疫荧光细胞染色,证明Neurexin、Neuroligin基因是否在肠神经系统表达,再采用免疫组织化学技术对20例先天性巨结肠狭窄段,移行段和扩张段肌间神经丛铺片标本染色,判断Neurexin、Neuroligin基因的表达情况。结果：(1)Neurexin、Neuroligin蛋白在小鼠、大鼠、豚鼠、人体的肠肌间神经丛与神经细胞标记Hu在同一细胞表达；而Neurexin蛋白与突触前标记物synaptophysin, Neuroligin蛋白与突触后标记物PSD95也在同一神经组织出现。(2) Neurexin、Neuroligin蛋白在先天性巨结肠扩张段表达最多,移行段明显减少(P0.05),病变段无表达；同一组织Neurexin和Neuroligin的表达无明显差异(P＞0.05)。结论：Neurexin、Neuroligin基因在鼠齿类动物及人体肠神经系统都有表达,不仅在胞体,而且在轴突、树突都有表达,Neurexin在突触前表达,Neuroligin在突触后表达。它们对肠神经系统突触的结构功能起重要作用,与一些胃、肠道动力性疾病有密切关系,是导致先天性巨结肠蠕动功能丧失的重要原因。
[Abstract]:Aim: to investigate the expression of Neurexin and Neuroligin genes in the intestinal nervous system of human and rodent, and to explore the expression characteristics and significance of ENS synaptic structure and function in Hirschsprung's disease (Hirschsprung's disease). Methods: first of all, we prepared mice, rats, guinea pigs and normal human intestinal intermuscular plexus. The expression of Neurexin Neuroligin gene in the enteric nervous system was proved by double immunofluorescence staining. Immunohistochemical staining was used to detect the expression of Neurexin Neuroligin gene in 20 specimens of myenteric plexus of Hirschsprung's disease. Results the expression of Neurexin Neuroligin protein in mouse, rat, guinea pig, human intestinal myenteric plexus and nerve cell labeled Hu was found in the same cell. Neurexin protein and presynaptic marker synaptophysin, Neuroligin protein and postsynaptic marker PSD95 were also found in the same nerve tissue.) Neurexin Neuroligin protein was most expressed in the dilated segment of Hirschsprung's disease. There was no significant difference between the expression of Neurexin and Neuroligin in the same tissue (P > 0.05). Conclusion the expression of Neurexin Neuroligin gene is found in both mouse dentin and human intestinal nervous system. It is expressed not only in the cell body but also in the axons and dendrites. Neurexin is expressed in presynaptic cells and neuroligin is expressed in postsynaptic system. They play an important role in the synaptic structure and function of the intestinal nervous system, and are closely related to some gastric and intestinal dynamic diseases, which are the important reasons for the loss of peristaltic function in Hirschsprung's disease.
【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R341

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