采用组织块移植法快速建立裸鼠原位骨肉瘤模型

发布时间：2018-06-02 05:32

本文选题：骨肉瘤 + 裸鼠　；参考：《中国组织工程研究》2015年49期

【摘要】：背景:骨肉瘤细胞起源于间叶组织这一特点使得骨肉瘤动物模型的构建存在较多困难,进展缓慢的问题。目的:采用组织块移植法建立裸鼠原位骨肉瘤模型,并观察和评价其生物学特性。方法:选用SPF级6周龄裸鼠15只,将制备好的MG-63骨肉瘤细胞悬液注射到裸鼠右前肢腋下,成瘤后连续传3代,待肿瘤生长稳定后将瘤块组织移植到裸鼠下肢胫骨髓腔内,评价其生物学特性,采用X射线观察裸鼠成瘤率和成瘤特点。并以正常裸鼠5只做对照。结果与结论:15只裸鼠造模过程顺利,造模后通过观察1只成瘤失败,成瘤率为93%;造模后观察发现采用组织块移植法三四周可见裸鼠下肢局部肿瘤状组织形成,四五周后X射线观察可见裸鼠胫骨中上段出现不明显的溶骨以及瘤样骨形成;骨肉瘤模型组碱性磷酸酶的表达水平明显高于正常对照组(P0.01)。数据表明采用组织块移植法进行裸鼠原位骨肉瘤造模,其造模方法简单,成瘤率高,瘤体生长速度快,对骨皮质及周围软组织的破坏力较强,接近临床骨肉瘤患者的实际情况。
[Abstract]:Background: the origin of osteosarcoma cells from mesenchymal tissue makes the construction of osteosarcoma animal model difficult and slow. Objective: to establish orthotopic osteosarcoma model in nude mice by tissue mass transplantation and to observe and evaluate its biological characteristics. Methods: 15 SPF grade 6 weeks old nude mice were selected. The prepared MG-63 osteosarcoma cell suspension was injected into the right forelimb of nude mice under axillary region, and the tumor was transferred for 3 consecutive generations. The tumor tissue was transplanted into the tibial medullary cavity of the lower extremity of nude mice after the tumor growth was stable. The biological characteristics were evaluated and the tumorigenic rate and characteristics of nude mice were observed by X-ray. 5 normal nude mice were used as control. Results and conclusion the process of modeling of 15 nude mice was smooth, and the tumorigenesis rate was 933 by observing the failure of tumorigenesis in 1 nude mouse model, and the local tumor tissue formation in lower extremity of nude mice was observed by tissue mass transplantation for three or four weeks. Four or five weeks later, the osteolysis and tumor-like bone formation were observed in the middle and upper tibia of nude mice, and the expression level of alkaline phosphatase in the osteosarcoma model group was significantly higher than that in the normal control group (P 0.01). The data showed that the orthotopic osteosarcoma model of nude mice was made by tissue mass transplantation. The method was simple, the tumorigenesis rate was high, the tumor growth rate was fast, and the damage to the bone cortex and surrounding soft tissue was stronger, which was close to the actual situation of the patients with clinical osteosarcoma.
【作者单位】：兰州大学第二医院骨三科;
【分类号】：R738.1;R-332

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