人脐带间充质干细胞诱导分化成脂细胞

发布时间：2018-06-02 17:04

本文选题：间充质干细胞 + 细胞分化　；参考：《中国输血杂志》2015年08期

【摘要】：目的观察人脐带间充质干细胞(human umbilical cord mesenchymal stem cells,HUC-MSCs)在体外向脂肪细胞诱导分化过程中细胞形态及表面抗原表达量变化。方法从脐带中分离培养间充质干细胞,至第3代,流式细胞仪检查其细胞表面抗原的表达量,加入成脂肪诱导剂体外诱导分化。诱导7 d,14 d,21 d,分别采用流式细胞仪分析细胞表面抗原的表达情况,结合油红O染色,观察细胞诱导分化情况。结果细胞培养至第3代,细胞表面表达CD90、CD44、CD29、CD71、CD73、CD105及CD13,不表达CD34、CD14、CD45、CD133、CD80、CD83、CD86、HLA-DR。成脂诱导7 d,细胞表面CD71表达量明显下降(P0.05),但其他一些重要的标志物仍处于高表达水平;诱导14 d细胞表面抗原的表达量下降更为明显,如CD44为65.21%,CD71为42.05%(P0.01),油红O染色有明显脂滴形成;诱导21 d细胞表面抗原的表达量均明显降低(P0.01),油红O染色胞浆中充满红色油滴。结论 HUC-MSCs成脂肪诱导剂作用下,可诱导成脂肪细胞,且细胞表面抗原的表达量会在诱导过程中发生相应的改变。
[Abstract]:Objective to observe the changes of morphology and surface antigen expression of human umbilical cord mesenchymal stem cells HUC-MSCs during differentiation into adipocytes in vitro. Methods Mesenchymal stem cells were isolated and cultured from umbilical cord to the third passage. The expression of surface antigens of mesenchymal stem cells was detected by flow cytometry, and differentiation was induced by adding fat inducer in vitro. The expression of cell surface antigen was analyzed by flow cytometry and the differentiation of cells was observed by oil red O staining. Results in the third passage of cell culture, CD90 CD441, CD29, CD71, CD71, CD73, CD105 and CD13 were expressed on the surface of the cells, and no CD34, CD14, CD14, CD135, CD133, CD80, CD83, CD86 or HLA-DR. On the 7th day after lipogenesis, the expression of CD71 on the cell surface decreased significantly, but some other important markers were still in high expression level, and the expression of surface antigen on the 14th day was more obvious. If CD44 was 65.21 and CD71 was 42.05, there were obvious lipid droplets in oil red O staining, and the expression of antigen on the surface of the cells decreased significantly at 21 days after induction, and the cytoplasm of oil red O staining was filled with red oil droplets. Conclusion Adipocytes can be induced by HUC-MSCs adipogenic inducer, and the expression of cell surface antigen will change in the course of induction.
【作者单位】：安徽省血液中心输血研究室;
【分类号】：R329.2

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