牛奶致小鼠便秘模型的建立与机制探讨

发布时间：2018-06-04 09:45

本文选题：牛奶 + 小鼠　；参考：《兰州大学》2009年硕士论文

【摘要】： 目的利用牛奶尝试建立一种制作简单、重复性好、基本符合STC临床及病理生理学特征的动物便秘模型,同时探讨其机理。方法以小鼠为实验对象,以浓缩奶为建模药物灌胃,观察实验动物的一般生物学指标、不排便动物数、首次排黑便时间、排便粒数及粪便总重量,小肠推进运动试验及肠道水分含量测定,通过在体结肠肌电测定和结肠慢波频率、振幅及c-kit抗体标记ICC阳性细胞面积测定,初步探讨便秘模型的建立方法以及形成机理。结果实验表明以70%牛奶饲养7天,可得符合要求的小鼠便秘模型。机制主要为:牛奶导致胃肠道酸碱平衡失调、小肠推进功能及肠道水分降低、c-kit抗体标记ICC阳性细胞面积减少、结肠肌电异常等。结论本研究建立的造模方法可行,符合原发性便秘的临床症状,尤其是慢传输型便秘。
[Abstract]:Objective to establish an animal model of constipation with simple reproducibility and accord with the clinical and pathophysiological characteristics of STC and to explore its mechanism. Methods mice were treated with concentrated milk as the model drug. The general biological indexes, the number of non-defecating animals, the first time of black defecation, the number of defecation grains and the total fecal weight of the experimental animals were observed. The method of establishing constipation model and the formation mechanism of constipation model were preliminarily discussed by intestinal propulsion exercise test and determination of intestinal water content, colonic electromyography, colon slow wave frequency, amplitude and area of ICC positive cells labeled with c-kit antibody. Results the experiment showed that the constipation model of mice could be obtained by feeding 70% milk for 7 days. The main mechanisms were as follows: gastrointestinal acid-base imbalance, intestinal propulsive function and intestinal water decrease, c-kit antibody labeled ICC positive cell area decreased, colon myoelectric abnormality and so on. Conclusion this method is feasible and consistent with the clinical symptoms of primary constipation, especially slow transit constipation.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R574.62;R-332

【参考文献】