不同方法刺激人外周血单个核细胞对SW1116杀伤作用的比较

发布时间：2018-06-04 16:24

本文选题：SW1116 + IL-2　；参考：《吉林大学》2008年硕士论文

【摘要】： 目的:通过不同方法刺激人外周血单个核细胞,比较其对结肠癌细胞SW1116的杀伤作用,为结肠癌的免疫治疗提供一定的理论依据。方法:肿瘤抗原、IL-2、CpG ODN刺激人外周血单个核细胞(效应细胞),然后与SW1116(靶细胞)共培养,分为抗原组、IL-2组、IL-2联合抗原组、CpG ODN组、CpG ODN联合抗原组及效应细胞对照组和靶细胞对照组。MTT法检测肿瘤细胞杀伤活性。结果:(1)肿瘤抗原组对SW1116细胞杀伤作用与效应细胞对照组比较无显著性差异;(2)IL-2组显著杀伤SW1116细胞,且IL-2联合抗原组对SW1116细胞的杀伤效应强于单独IL-2组或抗原组;(3)CpG ODN组亦明显杀伤SW1116细胞,CpG ODN联合抗原组对SW1116细胞的杀伤作用与单独CpG ODN组或抗原组比较差异有显著性,并且与IL-2联合抗原组相比亦有显著性差异。结论:IL-2或CpG ODN与肿瘤抗原联合对SW1116细胞杀伤作用强,且CpG ODN与肿瘤抗原联合对SW1116细胞杀伤效果更佳。
[Abstract]:Aim: to stimulate human peripheral blood mononuclear cells (PBMC) by different methods and compare their killing effects on colon cancer cells (SW1116) in order to provide a theoretical basis for immunotherapy of colon cancer. Methods: tumor antigen IL-2CpG ODN stimulated human peripheral blood mononuclear cells (PBMC) and co-cultured with SW1116 (target cells). The tumor cells were divided into antigenic group and IL-2 group combined with antigen group. The cytotoxic activity of tumor cells was detected by MTT assay in CpG ODN combined antigen group, effector cell control group and target cell control group. Results (1) there was no significant difference between tumor antigen group and effector cell control group in killing SW1116 cells. Moreover, the killing effect of IL-2 combined antigen group on SW1116 cells was stronger than that of IL-2 alone group or CpG ODN group. The killing effect of CpG ODN combined antigen group on SW1116 cells was significantly higher than that of CpG ODN group or antigen group alone. There was also significant difference compared with IL-2 combined antigen group. Conclusion the combination of tumor antigen and IL-2 or CpG ODN has strong killing effect on SW1116 cells, and the combination of CpG ODN and tumor antigen can kill SW1116 cells better.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R392

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