Cuff电极的构建与性能的初步研究

发布时间：2018-06-06 04:43

本文选题：神经电信号 + Cuff电极　；参考：《第四军医大学》2008年硕士论文

【摘要】： Cuff电极是一种植入到外周神经周围的电极,可以用来记录神经电信号,并可作为刺激电极用于神经肌肉功能性刺激( functional neuromuscular stimulation,FNS)。本研究中我们首先建立了一种Cuff电极的构建方法,并对其物理性能作了研究;然后将其作为一种神经信号记录电极,植入到狗的面神经分支周围,以探讨我们所构建的电极作为神经电极的可行性;最后通过分析记录到的信号的稳定性以及对神经的组织形态学的影响,研究应用该电极进行长期植入时的稳定性能。目的: 1.构建Cuff电极,并从物理性能方面研究其作为植入电极使用的可能性; 2.将构建的Cuff电极,植入到狗的面神经颧支周围,提取神经电信号,以研究这种电极能否作为提取外周神经电信号的电极使用; 3.将Cuff电极作为神经记录电极长期植入动物体内,从所提取到的神经电信号的稳定性,以及对植入电极组织形态的影响方面,观察是否可以作为长期植入电极使用? 方法: 1.使用预成套管缝合法构建Cuff电极,然后从电极的导电性与绝缘性两个方面测试其物理性能; 2.在狗面神经颧支周围植入Cuff电极,通过这个植入电极,提取神经信号并进行信号分析; 3.在狗面神经颧支周围植入Cuff电极,通过这个植入电极,分别在14天、6月后提取自然发生的神经电信号,比较分析不同时间提取的信号的差异性。通过组织形态学的方法,在Cuff电极植入后6个月观察分析周围有电极植入的神经段落的组织形态学变化,以分析植入电极对神经的影响。结果: 1.应用预成硅胶套管缝合法构建出了Cuff电极,测试结果显示其具有良好的导电性和绝缘性,可作为植入电极使用; 2.通过研究中所构建的Cuff电极可以提取到能反映神经所支配肌肉的功能作用状态的神经电信号,说明这种电极可以作为用来提取神经电信号的神经电极使用; 3.在电极植入后14天、6月均能提取出能反映眨眼动作发生的神经信号,所提取到的自然睁眼状态下的神经信号在根方均值RMS和中位频率MPF方面没有明显的变化。长期植入Cuff电极的神经,未发现有明显的损伤性形态改变,这说明该电极可以作为长期植入性电极进行应用。结论: 本研究提供了一种神经Cuff电极的制作方法,通过这种方法构建的神经Cuff电极,可以应用于神经电信号的研究和神经功能修复等领域。
[Abstract]:The Cuff electrode is a kind of electrode implanted around the peripheral nerve. It can be used to record the nerve signal and be used as the stimulation electrode to stimulate the (functional neuromuscular stimulation. In this study, we first established a method of constructing Cuff electrode and studied its physical properties, then used it as a kind of nerve signal recording electrode and implanted it around the facial nerve branch of dog. The feasibility of using the electrode as nerve electrode was discussed. Finally, the stability of the electrode was studied by analyzing the stability of the recorded signal and the effect on the histomorphology of the nerve. Objective: 1. Cuff electrode was constructed, and the possibility of using it as implanted electrode was studied from the aspect of physical properties. 2. The constructed Cuff electrode was implanted around the zygomatic branch of the facial nerve of the dog and the nerve signal was extracted to study whether the electrode could be used as the electrode to extract the peripheral nerve signal. 3. The Cuff electrode was used as the nerve recording electrode for a long time to be implanted into the animal body. From the stability of the extracted nerve signal and the effect on the tissue morphology of the implanted electrode, can it be used as the long-term implanted electrode? Methods: 1. The Cuff electrode was constructed by preformed casing suture method, and the physical properties of the electrode were tested from two aspects: electrical conductivity and insulation. 2. The Cuff electrode was implanted around the zygomatic branch of the facial nerve of the dog. The nerve signal was extracted and analyzed by the implanted electrode. 3. The Cuff electrode was implanted around the zygomatic branch of the facial nerve of the dog. After 14 days and 6 months, the naturally occurring nerve signals were extracted, and the differences of the signals extracted at different times were compared and analyzed. Histomorphology was used to observe and analyze the histomorphologic changes of peripheral nerve segments implanted with Cuff electrodes 6 months after implantation, in order to analyze the effect of implanted electrodes on nerves. Results: 1. The Cuff electrode was constructed by using preformed silica gel casing suture method. The test results show that the electrode has good conductivity and insulation and can be used as implanted electrode. 2. The constructed Cuff electrode can extract the nerve signal which can reflect the function state of the nerve innervated muscle, which indicates that the electrode can be used as the nerve electrode to extract the nerve signal. 3. After 14 days of electrode implantation, the nerve signals which can reflect blink action were extracted in 6 months. The nerve signals extracted under natural open eyes showed no significant changes in root mean RMS and median frequency MPF. No obvious morphological changes were found in the nerve implanted with Cuff electrode for a long time, which indicated that the electrode could be used as a long-term implanted electrode. Conclusion: This study provides a method for the fabrication of neural Cuff electrodes. The neural Cuff electrodes constructed by this method can be used in the research of neural electrical signals and nerve functional repair and so on.
【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R312

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