慢性不完全性睡眠剥夺后青春期大鼠睡眠特征及对空间性学习记忆的影响及分子机制

发布时间：2018-06-07 04:43

本文选题：睡眠剥夺 + 空间学习记忆　；参考：《复旦大学》2010年硕士论文

【摘要】：第一部分目的观察慢性不完全睡眠剥夺对青春期大鼠睡眠结构的影响,探讨睡眠剥夺后睡眠-觉醒周期有效恢复并保证睡眠结构稳态的最低睡眠时间。方法未成年雄性SD大鼠随机分为正常对照组(C)和4小时睡眠剥夺组(4hSD),予以轻触等方法进行人工不完全睡眠剥夺,连续7天。利用高度自动化睡眠觉醒解析平台记录分析大鼠睡眠的量、质及睡眠时相构成；同时观察正常幼年大鼠在发育过程中睡眠结构的变化。结果1每天4h夺眠,连续7天后青春期大鼠睡眠量增加以黑天的NREM增加为主；SWA、Power density增加,以白天为主；此外觉醒、NREM睡眠和REM睡眠的之间转换均降低,根据觉醒量明显下降可以得出睡眠以长时间NREM为主。 2在夺眠后第1天睡眠结构改变明显,以夺眠后各睡眠-觉醒时相持续时间和各时相转换变化明显,夺眠后第7天以Power density变化明显,而夺眠后第3天,夺眠后第5天睡眠反弹变化不明显。结论每天4h夺眠,连续7天可增加黑天的NREM睡眠量,增加白天的NREM和REM睡眠深度。第二部分目的观察不同时间的慢性不完全睡眠剥夺对青春期大鼠以及成年大鼠的空间学习记忆能力及机制,探讨不同时间的慢性不完全睡眠剥夺导致幼年和成年大鼠认知功能障碍和海马内腺苷水平、PDE等分子表达之间联系。方法青春期和成年雄性SD大鼠随机分为正常对照组(C)、2小时睡眠剥夺组(2h SD)、4小时睡眠剥夺组(4h SD)、6小时睡眠剥夺组(6h SD)以及8小时睡眠剥夺组(8h SD),连续夺眠7天。采用Morris水迷宫对所有大鼠进行空间认知功能训练,训练结束后予以轻触等方法进行人工不完全睡眠剥夺；利用Morris水迷宫的空间平台定位实验测定大鼠的空间记忆；运用RT-PCR技术测定大鼠海马腺苷受体以及PDE4的表达。结果1、各组大鼠夺眠后学习训练(认知功能)的潜伏期无明显变化,4周龄(4w)大鼠夺眠2h即出现上台前总路程增加,学习能力下降：2、4周龄大鼠夺眠6h后海马腺苷受体A1R,A2AR水平增加,4周龄大鼠夺眠8h PDE4水平增加,而8周龄大鼠无明显变化。结论每天夺眠2h、4h、8h,连续夺眠7天,即降低青春期大鼠的水迷宫空间学习能力,而对成年大鼠的学习能力无变化,每天夺眠2h、8h,连续夺眠7天,降低了成年大鼠的水迷宫空间记忆能力,而对青春期大鼠的记忆能力无变化；实时定量RT-PCR结果显示夺眠后7天,在夺眠6h组青春期大鼠A1,A2A受体升高,夺眠8h后青春期大鼠PDE4升高有明显意义。
[Abstract]:Part one Objective to observe the effect of chronic incomplete sleep deprivation on sleep structure of puberty rats, and to explore the minimum sleep time of sleep structure homeostasis after sleep deprivation. Methods male SD rats were randomly divided into normal control group (C) and 4-hour sleep deprivation group (4h SDN). The rats were subjected to artificial incomplete sleep deprivation for 7 days. The sleep quantity, quality and sleep phase composition of the rats were analyzed by using the highly automated sleep awakening analysis platform, and the changes of sleep structure during the development of normal young rats were observed at the same time. Results 1the amount of sleep in puberty rats increased after 4 hours of sleep capture every day, the increase of NREM in dark day was the main increase of SWAEM-Power density, and the change of sleep between NREM and REM sleep was decreased in Awakening and Awakening (Awakening). According to the obvious decrease in arousal, it can be concluded that sleep is mainly NREM for a long time. 2Sleep structure changed obviously on the first day after sleep capture, and the duration of each sleep-wake phase and the change of each time phase were obvious after sleep capture, Power density changed obviously on the 7th day after sleep capture, and on the third day after sleep capture. The change of sleep rebound was not obvious on the 5th day after sleep capture. Conclusion 4 h daily sleep deprivation can increase the amount of NREM sleep and NREM and REM sleep depth in dark day for 7 days. Part two Objective to observe the spatial learning and memory ability and its mechanism of chronic incomplete sleep deprivation in adolescent and adult rats at different times. To investigate the relationship between chronic incomplete sleep deprivation and expression of adenosine in hippocampus and cognitive dysfunction in young and adult rats. Methods male Sprague-Dawley rats were randomly divided into 2 hour sleep deprivation group (control group) and 8 hour sleep deprivation group. The spatial cognitive function of all rats was trained by Morris water maze, and the artificial incomplete sleep deprivation was carried out by using light touch after training, and the spatial memory of the rats was measured by the Morris water maze spatial platform positioning experiment. RT-PCR technique was used to detect the expression of adenosine receptor and PDE4 in rat hippocampus. Results 1. There was no significant change in the latency of learning training (cognitive function) after sleep capture in each group. The level of A1R A2AR in hippocampus was increased in rats of 4 weeks old, but the level of PDE4 in rats of 8 weeks old did not change. Conclusion the spatial learning ability of water labyrinth in adolescent rats was reduced after 2 hours of sleep for 4 hours and 8 hours for 7 consecutive days. However, there was no change in learning ability of adult rats. The rats were deprived of sleep for 2 hours and 8 hours a day for 7 consecutive days. The spatial memory ability of water maze in adult rats was decreased, but the memory ability of puberty rats was not changed. The results of real-time quantitative RT-PCR showed that the A1A2A receptor of adolescent rats increased 7 days after sleep deprivation. The increase of PDE4 in pubertal rats after 8 h sleep capture has significant significance.
【学位授予单位】：复旦大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R338

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