LPS激活巨噬细胞NLRP3炎性小体的作用研究

发布时间：2018-06-07 23:50

本文选题：脂多糖 + 巨噬细胞　；参考：《热带医学杂志》2015年04期

【摘要】：目的探讨脂多糖(LPS)激活巨噬细胞NLRP3炎性小体的作用。方法用佛波酯诱导人单核细胞(THP-1)分化为巨噬细胞。实验分为对照组、LPS组、LPS+NLRP3 si RNA组、LPS+Scrambled si RNA组。采用免疫荧光染色检测核苷酸结合寡聚化结构域样受体家族pyrin结构域蛋白3(NLRP3)和凋亡相关斑点样蛋白ASC的共定位、Western blotting检测NLRP3蛋白的表达、ELISA检测细胞培养上清白介素1β(IL-1β)的浓度。结果与对照组相比,NLRP3和ASC蛋白的共定位明显增多,LPS组NLRP3蛋白的表达增强。LPS还能使细胞培养上清IL-1β的浓度显著升高,NLRP3 si RNA干扰后细胞培养上清IL-1β的浓度显著降低。结论LPS可以促进炎性小体成分NLRP3、ASC的表达,激活NLRP3炎性小体,刺激巨噬细胞分泌炎症因子IL-1β。
[Abstract]:Objective to investigate the role of lipopolysaccharide (LPS) in activating inflammatory corpuscles of macrophage NLRP3. Methods Human monocytes were induced to differentiate into macrophages by phorbol ester. The experiment was divided into two groups: control group, LPS NLRP3 si RNA group and LPS scrambled si RNA group. The expression of NLRP3 protein in the supernatant of nucleotide binding oligodeoxyribonucleotide receptor family (pyrin domain protein 3nLRP3) and apoptosis-related dot-like protein (ASC) was detected by immunofluorescence staining. The expression of NLRP3 protein was detected by Elisa and the concentration of IL-1 尾 in the supernatant of cell culture was detected by Elisa. Results compared with the control group, the co-localization of NLRP3 and ASC protein was significantly increased. The expression of NLRP3 protein in LPS group was enhanced. LPS could also significantly increase the concentration of IL-1 尾 in the supernatant of cell culture and decrease the concentration of IL-1 尾 in cell culture supernatant after the interference of NLRP3 si RNA. Conclusion LPS can promote the expression of NLRP3ASC, activate the inflammatory corpuscles of NLRP3, and stimulate macrophages to secrete the inflammatory factor IL-1 尾.
【作者单位】：中山大学中山医学院病理生理教研室;
【基金】：国家自然科学基金(81370371)
【分类号】：R392

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