Nur77在不同发育阶段小鼠睾丸的表达及其功能

发布时间：2018-06-09 03:23

本文选题：Nur + 睾酮　；参考：《中国男科学杂志》2015年04期

【摘要】：目的观察核受体Nur77在不同发育阶段小鼠睾丸的表达,并探讨其对睾酮合成的作用。方法用1周龄的ICR雄性小鼠作为幼年期组(相当于人类幼年期,n=6),4周龄的ICR雄性小鼠作为性发育前组(相当于人类青春期前,n=6),12周龄的ICR雄性小鼠作为性成熟期组(相当于人类青壮年,n=6),12月龄的ICR雄性小鼠作为老年组(相当于人类中老年,n=6)。Real time PCR和Western Blot分别检测小鼠睾丸中Nur77 mRNA和蛋白水平表达。体外培养小鼠睾丸间质细胞系MLTC-1,Nur77干涉腺病毒(Ad-siNur77)及空载腺病毒(Ad-CMV)转染MITC-1。放射免疫法检测MLTC-1细胞培养液上清的睾酮含量,Real time pCR和Western Blot检测StAR及HSD3B的表达。结果 Nur77的mRNA水平在青春期前表达量最高,蛋白水平则在性成熟期小鼠睾丸中最高;Nur77干涉后MLTC-1细胞的睾酮合成含量较末干涉组明显降低;Nur77干涉后MLTC-1细胞的StAR和HSD3B的表达较未干涉组明显降低。结论 Nur77在小鼠睾丸组织的表达随年龄变化而变化,这种变化对于维持小鼠体内的睾酮水平可能有重要意义。
[Abstract]:Objective To observe the expression of nuclear receptor Nur77 in mouse testis at different developmental stages and to explore its role in the synthesis of testosterone. Methods the 1 week old ICR male mice were used as the young group (equivalent to human young, n=6) and 4 weeks old as the predevelopmental group (equivalent to pre puberty, n=6), and 12 weeks old ICR male mice. For the sexual maturity group (equivalent to human youth, n=6), 12 month old of the ICR male mice were used as the elderly group (equivalent to human medium and old age, n=6).Real time PCR and Western Blot to detect the expression of Nur77 mRNA and protein in mice testicles. Ad-CMV transfected MITC-1. radioimmunoassay to detect the testosterone content in the supernatant of MLTC-1 cell culture, Real time pCR and Western Blot to detect the expression of StAR and HSD3B. Results Nur77 mRNA level was highest before puberty, and the protein level was highest in the testis of sexual maturity. Compared with the end interference group, the expression of StAR and HSD3B in MLTC-1 cells after Nur77 interference was significantly lower than that in the non interference group. Conclusion the expression of Nur77 in mouse testis changes with age, which may be of great significance in maintaining the level of testosterone in mice.
【作者单位】：东南大学附属中大医院中西医结合男科;南京大学附属鼓楼医院生殖中心;
【基金】：南京中医药大学青年自然科学基金(12XZR13) 国家自然科学基金青年基金(81302969);国家自然科学基金(81273760);国家自然科学基金(81473678)
【分类号】：R339.21

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