肿瘤冻融抗原、CpGODN和IL-2共同刺激诱导人外周血单个核细胞增殖活性及对SW1116的杀伤活性的研究

发布时间：2018-06-09 04:46

本文选题：肿瘤冻融抗原 + CpG　；参考：《吉林大学》2008年博士论文

【摘要】： 本实验是以人外周血单个核细胞为效应细胞,以结肠癌细胞株SW1116为靶细胞,以CpGODN和IL-2作为免疫佐剂,应用肿瘤冻融抗原预刺激来使人免疫细胞致敏,观察免疫细胞增殖情况和肿瘤杀伤作用,另外我们对比观察了肿瘤病人和健康志愿者的外周血单个核细胞对这种免疫激活的反映情况。结果表明:我们单独应用肿瘤冻融抗原、CpGODN和IL-2均可刺激人外周血单个核细胞而促进其增殖增强其肿瘤杀伤作用,而联合应用肿瘤冻融抗原、CpGODN和IL-2共同刺激能够强烈刺激人外周血单个核细胞,促进其增殖,提高其肿瘤杀伤作用,与相应对照组(空白对照、单独应用三者之一或联合应用其中任意两种)相比均有显著差异(P0.01);流氏细胞仪检测发现肿瘤冻融抗原、CpGODN和IL-2共刺激组T淋巴细胞CD4+和CD8+亚群均明显增殖,二者比例无明显改变,CD3+表达也明显增强,与相应对照组相比,差异有显著性(P0.05);共刺激组效应细胞的增殖活性、T细胞亚群的活性表达、肿瘤杀伤率与加入佐剂CpG ODN的浓度呈正相关;肿瘤病人的外周血单个核细胞同样可被我们的方法激活而表达较强的增殖活性和肿瘤杀伤作用与正常人相应实验组相比无显著差异(P0.05)。
[Abstract]:In this experiment, human peripheral blood mononuclear cells were used as effector cells, colon cancer cell line SW1116 as target cells, CpG ODN and IL-2 as immune adjuvants, and tumor freeze-thawed antigen prestimulation was used to sensitize human immune cells. The immune cell proliferation and tumor killing activity were observed. In addition, the response of peripheral blood mononuclear cells (PBMC) of tumor patients and healthy volunteers to this immune activation was compared. The results showed that CpG ODN and IL-2 alone could stimulate the proliferation of human peripheral blood mononuclear cells and enhance their tumor killing effect. The combination of CpG ODN and IL-2 could stimulate human peripheral blood mononuclear cells (PBMC), promote its proliferation and enhance its tumor killing effect. There was a significant difference between one of the three groups alone or any of the two groups (P 0.01), and the proliferation of CD4 and CD8 subsets of T lymphocytes was found in CpG ODN and IL-2 co-stimulation group by flow cytometer, and the results showed that the proliferation of CD4 and CD8 subsets of T lymphocytes was significantly increased in CpG ODN and IL-2 co-stimulation groups. There was no significant change in the expression of CD3 in the two groups, and there was a significant difference in the expression of CD3 between the two groups (P 0.05), and the activity expression of T cell subsets in the co-stimulation group. The tumor killing rate was positively correlated with the concentration of the adjuvant CpG ODN. The peripheral blood mononuclear cells of tumor patients could also be activated by our method and expressed strong proliferative activity and tumor killing effect.
【学位授予单位】：吉林大学
【学位级别】：博士
【学位授予年份】：2008
【分类号】：R392

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