腹腔注射脂多糖联合缺氧在脑瘫模型评价的实验研究

发布时间：2018-06-11 17:34

本文选题：炎症 + 缺血缺氧　；参考：《新疆医科大学》2009年硕士论文

【摘要】： 目的:探讨孕鼠宫内感染合并缺氧致幼鼠脑瘫的模型制作及评价方法。方法:实验动物分为三组:(1)脂多糖+缺氧组:选用孕16～18天健康Wistar雌性大鼠脂多糖腹腔注射(0.4mg/kg),12小时后置于缺氧环境中缺氧2.5小时,4小时后再次腹腔注射脂多糖(0.4mg/kg);(2)颈动脉结扎+缺氧组:组出生后第7天Wistar幼鼠结扎右侧颈动脉结合缺氧环境的方法制作幼鼠脑瘫模型;(3)对照组:仅行颈正中切口,然后缝合切口。于术后4周对三组实验动物进行足印分析试验、股四头肌复合肌肉运动诱发电位及病理学检测。结果:(1)足印分析试验:脂多糖+缺氧组和颈动脉结扎+缺氧组实验鼠前后肢足印重复性差,表现为间距较大,且间距不稳定,明显大于对照组(P0.05);(2)两实验组动物后肢股四头肌CMAP与对照组相比,引出最大波幅所需的刺激强度增大,引出波幅降低(P0.05); (3)HE染色光镜观察两实验组脑室旁正常组织结构破坏,细胞排列紊乱,局部大小不等圆形软化灶,在颈动脉结扎+缺氧组以损伤侧脑室周围变化明显。结论:应用孕鼠宫内感染合并缺氧的方法制作幼鼠脑瘫模型稳定可靠,此模型与成熟的单侧颈动脉结扎缺氧模型相比具有微创的、简单易行、逼真的模拟人类在怀孕晚期宫内感染缺氧致胎儿脑部损伤的优点,值得推广。
[Abstract]:Objective: to investigate the model of cerebral palsy induced by intrauterine infection and hypoxia in pregnant rats. Methods: the experimental animals were divided into three groups: lipopolysaccharide hypoxia group: lipopolysaccharide (LPS) was injected intraperitoneally into female Wistar rats at gestation 1618 days after 12 hours of hypoxia for 2.5 hours and 4 hours. Artery ligation hypoxia group: right carotid artery ligation combined with anoxic environment in Wistar young rats on the 7th day after birth (n = 3) the model of cerebral palsy in young rats was established by anoxia) control group: the median cervical incision was used only, the right carotid artery was ligated on the 7th day after birth. Then suture the incision. Four weeks after operation, three groups of experimental animals were tested with foot print analysis, quadriceps femoris combined muscle motor evoked potential and pathological examination. Results (1) footprint analysis test: rats in lipopolysaccharide hypoxia group and carotid artery ligation hypoxia group had poor reproducibility of foreand hindlimb footprints, which were characterized by large spacing and unstable spacing. Compared with the control group, CMAP of the quadriceps femoris of the hind limbs of the two experimental groups increased the stimulation intensity needed to elicit the maximum wave amplitude, and decreased the amplitude of the CMAP, and the damage of the normal tissue structure around the ventricle of the two experimental groups was observed under light microscope. The cells were disordered, the local size was not equal to round softening foci, and the changes were obvious in the group of carotid artery ligation and hypoxia. Conclusion: the model of cerebral palsy in young rats made by intrauterine infection combined with hypoxia in pregnant rats is stable and reliable. Compared with the mature unilateral carotid artery ligation hypoxia model, this model is minimally invasive and easy to perform. The advantages of anoxic fetal brain injury caused by anoxia in late pregnancy are worth popularizing.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R742.3;R-332

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