编码糖蛋白B的裸基因疫苗诱导单纯疱疹病毒Ⅰ型感染小鼠的体液和细胞免疫

发布时间：2018-06-13 03:24

本文选题：疫苗 + DNA　；参考：《延边大学医学学报》2010年03期

【摘要】：[目的]用编码单纯疱疹病毒Ⅰ型(HSV-1)糖蛋白B的pDNA转染小鼠,研究基因疫苗治疗感染性疾病的有效性,并分析免疫反应.[方法]采用血管内注射方法,将含有不同剂量、不同pDNAs的生理盐水注入Balb/c小鼠体内,1周后行再次免疫,观察以不同组合pDNA进行免疫的实验组和对照组小鼠被致死剂量HSV-1感染后生存率间的差异.通过潜伏感染的检测、血清中细胞因子水平的测定、细胞毒性反应的测定及中和实验评价免疫效果.[结果]接种pG.gB或接种pGEG.gB的小鼠,与相应对照组比较,生存率和诱导产生中和抗体的效价与剂量呈正相关,pGEG.gB免疫组对P815.gB细胞有显著的细胞毒性作用,pGEG.gB免疫小鼠的脾细胞增殖反应显著强于pG.gB免疫的小鼠;pG.gB和pGEG.gB免疫后的小鼠血清中IL-12和IFN-γ水平均显著升高.[结论]裸pDNA的经小鼠尾静脉免疫可诱导HSV-1感染小鼠的体液和细胞免疫应答,对小鼠HSV-1急性感染有明显的预防和治疗作用,且含有EBNA1和oriP的EBV质粒载体明显增强CTL活性和增殖反应.
[Abstract]:[objective] to study the efficacy of gene vaccine in the treatment of infectious diseases by transfection of pDNA encoding herpes simplex virus type I (HSV-1) glycoprotein B in mice, and to analyze the immune response. [methods] by intravascular injection, saline containing different doses and different pDNAs was injected into Balb / c mice for 1 week and then immunized again. The survival rate of mice immunized with different combinations of pDNA by lethal dose HSV-1 was observed. The immune effect was evaluated by the detection of latent infection, the level of cytokines in serum, the determination of cytotoxic reaction and neutralization experiment. [results] the mice inoculated with pG.gB or pGEG.gB were compared with the control group. Survival rate and the titer of inducing neutralizing antibody were positively correlated with the dose of pGEG.gB immunized mice. The cytotoxicity of pGEG.gB immunized mice to P815.gB cells was significantly stronger than that of pGEG.gB immunized mice after pG.gB and pGEG.gB immunized mice. The levels of IL-12 and IFN- 纬 in serum of mice were significantly increased. [conclusion] Naked pDNA immunization via tail vein can induce humoral and cellular immune responses in mice infected with HSV-1, and has obvious preventive and therapeutic effects on acute HSV-1 infection in mice. And EBV plasmid vector containing EBNA1 and OLP significantly enhanced CTL activity and proliferation response.
【作者单位】：延边大学医学部;延边大学基础医学院免疫学与病原生物学教研部;
【基金】：国家自然科学基金项目号:3660170
【分类号】：R392

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