HaCaT细胞诱导MSCs向表皮干细胞分化机制的研究

发布时间：2018-06-14 13:01

  本文选题：骨髓间充质干细胞 + 表皮干细胞　； 参考：《中南大学》2009年硕士论文

【摘要】： 目的:研究HaCaT细胞诱导MSCs向表皮干细胞分化的机制。 方法:1密度梯度离心法分离、培养MSCs,免疫组织化学法鉴定CD34,CD44和CD45。2用插入式细胞培养器将HaCaT和MSCs共培养,设为实验组;MSCs单独培养,设为对照组。分别于第3和第7天免疫组织化学法鉴定CK19,Pan-ck和β1整合素。3试验分为3组,分别为共培养细胞上清液组,HaCaT上清液组和MSCs上清液组。ELISA法检测上清液中EGF含量。 结果:1分离出的细胞呈梭形,免疫组织化学染色CD34,CD45表达阴性,CD44表达阳性。2共培养后MSCs免疫组织化学染色CK19,Pan-ck和β1整合素阳性,共培养7天后MSCs CK19,Pan-ck和β1整合素的阳性率均高于共培养3天的(P＜0.05)。3共培养组上清液中EGF均值高于HaCaT组(P＜0.01)。 结论:1 HaCaT细胞可以诱导MSCs向表皮干细胞分化。2 EGF是HaCaT细胞诱导MSCs向表皮干细胞分化的因素之一。
[Abstract]:Objective: to study the mechanism of differentiation of MSCs into epidermal stem cells induced by HaCaT cells. Methods MSCs were isolated and cultured by the density gradient centrifugation method of 1: 1. CD34T CD44 and CD45.2 were identified by immunohistochemical method. HaCaT and MSCs were co-cultured in a plug-in cell culture apparatus. The MSCs were cultured separately in the experimental group and in the control group. On the 3rd and 7th day, CK19 Pan-ck and 尾 1 integrin 3 test were divided into three groups, the supernatant group of co-cultured cells, HaCaT supernatant group and MSCs supernatant group. Elisa was used to detect the content of EGF in supernatant. Results the cells isolated from 1 / 1 were fusiform. The positive expression of CD34, CD45, CD44, CD44, and 尾 1 integrin in MSCs were detected by immunohistochemistry, and the positive expression of CD44 and 尾 1 integrin in MSCs were detected by immunohistochemistry. The positive rates of CK19 Pan-ck and 尾 1 integrin in MSCs co-cultured for 7 days were higher than those in HaCaT group (P < 0.05). Conclusion the differentiation of MSCs into epidermal stem cells induced by 1: 1 HaCaT cells. 2 EGF is one of the factors that induce MSCs to differentiate into epidermal stem cells.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R329

【参考文献】

相关期刊论文 前10条

1 都义日,付小兵,李存保,孙同柱,方利君;表皮细胞生长因子在体诱导猪骨髓间充质干细胞向皮肤组织分化作用的研究[J];创伤外科杂志;2005年02期

2 朱国俊;白晓东;付小兵;;骨髓间充质干细胞与信号转导研究进展[J];感染.炎症.修复;2004年03期

3 王蓓,汪维伟;间充质干细胞的研究进展[J];国外医学.外科学分册;2005年06期

4 颉玉欣,王旭,张进贵;间充质干细胞的研究进展[J];河北医药;2005年02期

5 姜任武;王汉群;周昌宁;龙剑虹;;兔骨髓间质干细胞组织工程化肌腱模型的构建[J];南华大学学报(医学版);2008年03期

6 ;Full-thickness tissue engineered skin constructed with autogenic bone marrow mesenchymal stem cells[J];Science in China(Series C:Life Sciences);2007年04期

7 武晓静,黄岚,周骐,崔斌,董红梅,周音频;不同诱导条件对人骨髓基质干细胞向内皮分化的影响[J];中国动脉硬化杂志;2005年04期

8 杨锋;李易;卢竞前;王洁;李绍龙;;两种培养方法在猪自体骨髓间充质干细胞培养的比较研究[J];昆明医学院学报;2008年01期

9 郑朝晖,朱平,王彦宏,樊春梅,丁进,尚鹏;体外诱导骨髓间充质干细胞向软骨细胞的定向分化及其鉴定[J];细胞与分子免疫学杂志;2005年01期

10 吕春荣;叶绍辉;李林凤;张艳艳;顾恩妍;关伟军;马月辉;;表皮干细胞的鉴定方法[J];中国组织工程研究与临床康复;2008年43期

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