大鼠原位肝移植模型的建立

发布时间：2018-06-16 17:50

本文选题：二袖套法 + 大鼠　；参考：《中国组织工程研究》2012年05期

【摘要】：背景:"二袖套法"原位肝移植模型的建立缩短了无肝期的时间,并且显著提高了老鼠肝移植后的存活率。目的:在"二袖套法"的基础上,结合一些相关文献,建立稳定大鼠原位肝移植模型。方法:用改进的二袖套法对75对SD大鼠行原位肝移植,移植中门静脉、肝下下腔静脉用袖套法进行吻合,肝上下腔静脉用缝合法吻合,胆道采用支架法进行胆道重建。结果与结论:受体大鼠移植后一般状况良好,50例正式实验移植后1d存活率94%,1周存活率为90%。供肝热缺血时间均接近0,供体手术时间(34.44±3.25)min,受体手术时间(49.07±4.93)min,无肝期(17.26±2.51)min,下腔静脉平均阻断时间约为20min。说明只有熟练地掌握手术技巧,细致耐心的操作,最大程度减少各种并发症的发生,才能获得稳定的原位肝移植模型。
[Abstract]:Background: the establishment of a two-cuff orthotopic liver transplantation model shortens the time of anhepatic phase and significantly improves the survival rate after liver transplantation in mice. Objective: to establish a stable orthotopic liver transplantation model based on the two cuff method and some related literatures. Methods: 75 Sprague-Dawley rats underwent orthotopic liver transplantation with modified two-cuff method. Portal vein and inferior hepatic vena cava were anastomosed with cuff method, inferior hepatic vena cava was sutured and biliary tract was reconstructed by stenting. Results and conclusion: the 1-day survival rate of the recipient rats after transplantation was 94% and 90% respectively. The mean time of donor operation was 34.44 卤3.25 min, the donor operation time was 49.07 卤4.93 min, the anhepatic phase was 17.26 卤2.51 min, and the mean occlusion time of inferior vena cava was about 20 min. The results showed that only by mastering surgical skills and careful and patient operation, the occurrence of various complications could be minimized and a stable orthotopic liver transplantation model could be obtained.
【作者单位】：江西省人民医院器官移植科;
【基金】：江西省主要学科学术和技术带头人培养计划任务书(2008DD01100),课题名称:活体肝移植的基础和临床应用研究~~
【分类号】：R657.3;R-332

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