脱氢表雄酮诱导多囊卵巢综合征大鼠模型的表型研究

发布时间：2018-06-16 23:46

本文选题：脱氢表雄酮 + 多囊卵巢综合征　；参考：《世界中西医结合杂志》2015年11期

【摘要】：目的运用脱氢表雄酮(DHEA)诱导SD大鼠多囊卵巢综合征模型,研究模型大鼠的生殖表型和代谢表型。方法选取23日龄雌性SD大鼠,模型组每日颈背部皮下注射DHEA 6 mg/100 g体重,对照组每日颈背部皮下注射0.2 ml注射油剂。注射20 d后观察两组大鼠动情周期、卵巢形态学、性激素、体重、脂肪湿重、卵巢体积、OGTT血糖、空腹胰岛素、胰岛素抵抗指数(HOMA-IR)及血脂等生殖及代谢相关指标的变化。结果模型组大鼠失去规律的动情周期;卵巢体积增大,呈多囊样变;血清促卵泡生成素(FSH)、促黄体生成素(LH)、睾酮(T)水平明显高于对照组;糖耐量异常,空腹胰岛素及HOMA指数明显高于对照组;血清胆固醇、脂肪湿重、脂体比明显高于对照组。结论 DHEA可诱导SD大鼠出现与PCOS患者相似的生殖和代谢异常的改变,是研究多囊卵巢综合征生殖及代谢异常的较理想的动物模型。
[Abstract]:Objective to use dehydroepiandrosterone (DHEA) to induce polycystic ovary syndrome (PCOS) model in SD rats and study the reproductive phenotype and metabolic phenotype of the model rats. Methods 23 day old female SD rats were selected. The model group was injected with DHEA 6 mg/100 G on the back of the neck on the back of the day, and the control group was injected with 0.2 ml injections under the skin of the neck of the back daily. The two groups were observed after 20 D injection. Rats' estrous cycle, ovarian morphology, sex hormone, body weight, fat wet weight, ovarian volume, OGTT blood sugar, fasting insulin, insulin resistance index (HOMA-IR) and blood lipid and other reproductive and metabolic indexes were changed. Results the rats in the model group lost the regular estrous cycle, the ovarian volume increased, the polycystic change, and the serum follicle stimulating hormone (FSH), The level of luteinizing hormone (LH) and testosterone (T) was significantly higher than that of the control group. The abnormal glucose tolerance, fasting insulin and HOMA index were significantly higher than those of the control group. The serum cholesterol, fat wet weight and lipid body ratio were significantly higher than those of the control group. Conclusion DHEA can induce the abnormal changes of reproduction and metabolism similar to those of the PCOS patients, which is the study of the polycystic ovary syndrome. Ideal animal models for reproductive and metabolic abnormalities.
【作者单位】：河南中医学院第三临床医学院;
【基金】：河南中医学院博士科研基金项目(BSJJ2010-32)
【分类号】：R-332;R711.75

【参考文献】