小鼠骨髓间充质干细胞体外培养及其向成骨细胞诱导分化的实验研究

发布时间：2018-06-17 03:36

本文选题：骨髓间充质干细胞 + 细胞培养　；参考：《大连医科大学》2009年硕士论文

【摘要】： 目的:研究小鼠骨髓间充质干细胞(mesenchymal stem cells,MSCs)体外培养和扩增及定向诱导分化为成骨细胞的可能性和方法。方法:取C57BL小鼠双侧股骨和胫骨骨髓细胞进行体外培养,贴壁细胞传代。取第5代细胞诱导培养,倒置显微镜下观察其形态。取诱导培养14天后的细胞进行碱性磷酸酶染色和诱导培养21天后的细胞进行vonkossa矿化结节染色。结果:体外培养的MSCs生长较好,原代和传代细胞都具有很强的增殖能力,诱导培养后,ALP检测和vonkossa矿化结节染色均呈阳性反应。结论:MSCs能于体外培养存活和扩增,并且在一定条件下可以诱导分化为成骨细胞,可用作骨组织工程的种子细胞。
[Abstract]:Aim: to study the possibility and method of osteoblast differentiation from mesenchymal stem cells (MSCs) derived from mouse bone marrow mesenchymal stem cells (MSCs) in vitro. Methods: bone marrow cells from bilateral femur and tibia of C57BL mice were cultured in vitro and the adherent cells were subcultured. The cells of the fifth passage were cultured and the morphology of the cells was observed under inverted microscope. The cells cultured for 14 days were stained with alkaline phosphatase and those cultured for 21 days were stained with vonkossa mineralized nodules. Results: MSCs grew well in vitro and both primary and passage cells had strong proliferative ability. After induction and culture, both ALP detection and vonkossa mineralized nodule staining showed positive reaction. Conclusion\
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R329

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