肝郁血瘀型宫环出血病病症结合大鼠模型的建立及研究

发布时间：2018-06-19 03:53

本文选题：宫环出血 + 肝郁血瘀　；参考：《湖南中医药大学》2010年硕士论文

【摘要】：目的：本研究在中医理论指导下,探讨肝郁血瘀型宫环出血病证动物模型的建立方法,并在此基础上进行发病机理的研究。方法：将大鼠随机分为4组：空白组、置环组、模型组、治疗组。通过长期慢性束缚应激结合孤养建立起应激大鼠的肝郁症模型,观察分析在模型建立过程中实验动物的体重、蔗糖水摄入量以及行为学指标方面的变化,进行分析,寻找合适的动物模型建立方法。本研究应用一种全新的蛋白组学技术——抗体芯片技术测量动物模型子宫组织细胞因子水平。分析体重、蔗糖摄入量、行为与肝郁症的相关关系,探讨细胞因子在肝郁血瘀型宫环出血病病理生理机制中的作用,观测治疗药物对这些指标的影响以佐证模型。结果：模型组大鼠行为异常,主要表现为躲避、活动减少、大便松散、皮色失去光泽,生长较对照组缓慢(P0.05)。糖水偏好实验显示造模过程中模型组对糖水的偏好摄入少于对照组(P0.05)。与置环组相比,肝郁血瘀+置环模型组有4种因子的水平发生了显著变化。其中睫状神经营养因子(CNTF)水平同置环组相比降低(P0.05),白介素-1β(IL-1β)、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平较置环组显著升高(P0.05)。结论：金刃损伤胞宫是宫环出血关键病因,肝气郁结,瘀血阻络为其主要发病机理。本试验从病因学、行为学和病理表现上用慢性束缚的方法成功构建了肝郁证大鼠模型。研究提示,抗体芯片技术能够高通量、平行性检测大鼠子宫多种细胞因子的表达水平,CNTF、IL-1β、IL-6、TNF-α可能与肝郁症发病机制有关。抗体芯片技术有可能成为中医症候学研究的新型技术平台。
[Abstract]:Objective: under the guidance of traditional Chinese medicine theory, this study explored the establishment of animal model of uterine ring hemorrhage syndrome of liver stagnation and blood stasis, and studied the pathogenesis on the basis of it. Methods: rats were randomly divided into 4 groups: blank group, ring setting group, model group and treatment group. The liver depression model of stress rats was established by long-term chronic restraint stress combined with solitary rearing. The changes of body weight, sucrose water intake and behavioral indexes of experimental animals were observed and analyzed during the establishment of the model. To find a suitable method for establishing animal model. In this study, a novel proteomics technique, antibody chip technique, was used to measure cytokine levels in uterine tissues of animal models. The relationship between body weight, sucrose intake, behavior and liver depression was analyzed. The role of cytokines in the pathophysiological mechanism of uterine circulation hemorrhage with liver depression and blood stasis was discussed. The effects of therapeutic drugs on these indexes were observed to prove the model. Results: the rats in the model group showed abnormal behavior, such as avoidance, decreased activity, loose stool, lost luster of skin color and slower growth than that of the control group (P 0.05). Sugar water preference experiment showed that the preference intake of sugar water in model group was less than that in control group (P 0.05). Compared with the ring setting group, there were significant changes in the level of four factors in the model group of liver stagnation and blood stasis. The level of ciliary neurotrophic factor CNTF- 伪 was significantly higher than that of the ring setting group (P 0.05), and the levels of IL 1 尾, IL 1 尾, IL 6 and TNF- 伪 were significantly higher than those in the ring setting group. Conclusion: the damage of the uterus is the key cause of uterine ring hemorrhage, the main pathogenesis is stagnation of liver-qi and obstruction of collaterals by blood stasis. In this study, the rat model of liver depression was successfully established by chronic restraint in etiology, behavior and pathology. It was suggested that the antibody chip technique could be used to detect the expression level of multiple cytokines in rat uterus in parallel mode. The results suggested that IL-6 TNF- 伪 might be related to the pathogenesis of liver depression. Antibody chip technology may become a new technology platform for TCM syndrome research.
【学位授予单位】：湖南中医药大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R-332;R271.9

【引证文献】