小鼠3个不同部位巨噬细胞的分离培养与鉴定

发布时间：2018-06-20 01:04

本文选题：小鼠 + 巨噬细胞　；参考：《预防医学情报杂志》2015年11期

【摘要】：目的建立小鼠3种不同部位巨噬细胞体外分离培养的方法,并对其进行鉴定。方法分别从小鼠腹腔、脾脏、骨髓中分离获取巨噬细胞,经台盼蓝染色计数后于含10%胎牛血清的DMEM培养液、1%小牛血清的RPMI-1640培养基中培养;通过活细胞形态观察;HE染色光镜观察;吞噬鸡红细胞功能检测;酸性磷酸酶及非特异性酯酶染色测定细胞内酶的表达等生物学技术鉴定培养的贴壁细胞性质。结果获得高纯度的巨噬细胞,具有巨噬细胞的形态特征,具备良好的吞噬功能,鸡红细胞吞噬率分别为腹腔94.68%,脾脏92.47%,骨髓93.11%;酸性磷酸酶染色阳性率为腹腔89.43%,脾脏85.21%,骨髓88.55%;α-醋酸奈酚酯酶染色阳性率为腹腔85.32%,脾脏81.81%,骨髓85.76%。。结论小鼠3个不同部位均可简便实用的分离出巨噬细胞,可以满足不同的研究目的。
[Abstract]:Objective to establish a method of isolation and culture of macrophages from 3 different parts of mice in vitro, and to identify it. Methods the macrophages were isolated from the abdominal cavity, spleen and bone marrow of mice. After trypan blue staining, the DMEM culture medium containing 10% fetal bovine serum and 1% calf serum were cultured in RPMI-1640 medium. Inspection; HE staining light microscopy, phagocytosis of chicken red cell function detection, acid phosphatase and nonspecific esterase staining to determine the properties of the adherent cells cultured by biological techniques. The results obtained high purity macrophages, phagocytic characteristics, good phagocytosis and phagocytosis rate of chicken red cells. 94.68% of the abdominal cavity, 92.47% of spleen and 93.11% bone marrow, the positive rate of acid phosphatase staining was 89.43% in the abdominal cavity, 85.21% in the spleen and 88.55% in the bone marrow, and the positive rate of alpha acetate esterase acetate esterase was 85.32% in the abdominal cavity and 81.81% in the spleen, and the bone marrow 85.76%.. Conclusion that 3 different parts of the mice can be easily and practically separated from the macrophages, which can meet the different research. Objective.
【作者单位】：咸阳市疾病预防控制中心;陕西师范大学附属中学分校;
【分类号】：R392

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