经硬式鼻腔镜气管内滴注化学制剂建立AECOPD动物模型

发布时间：2018-06-22 12:08

本文选题：慢性阻塞性肺疾病急性加重 + 动物模型　；参考：《辽宁中医药大学学报》2015年11期

【摘要】：目的:寻求一种在损伤最小的情况下建立慢性阻塞性肺病急性加重期(AECOPD)大鼠模型的新方法。方法:将40只大鼠分为正常对照组、模型组,乙醚麻醉后,在硬式鼻腔镜引导及松下摄录监视系统下,使用自制鼠麻醉喉镜,气管内隔周定期注入弹性蛋白酶,并最后滴入细菌,从而建立AECOPD大鼠模型。结果:模型组大鼠精神萎靡,动则气喘,呼吸深大,气道分泌物增多;肺组织体积增大、出现瘀血,病理结果显示肺泡壁变薄变大,肺泡腔融合增大,部分破裂融合形成肺大泡,肺泡数目明显减少,肺泡周围可见炎细胞浸润,改变与人类COPD改变相符合。模型组大鼠肺功能FEV0.3、FEV0.3/FVC、FVC较正常对照组显著下降。结论:通过运用硬式鼻腔镜、松下摄录监视系统及自制鼠麻醉喉镜,气管内滴入弹性蛋白酶,并结合细菌攻击可以成功建立AECOPD大鼠模型。
[Abstract]:Aim: to explore a new method for establishing acute exacerbation of chronic obstructive pulmonary disease (AECOPD) rat model with minimal injury. Methods: forty rats were divided into normal control group and model group. Under the guidance of hard nasal cavity and under the surveillance system of subarachnoid recording, elastase was injected into the trachea every other week with homemade anesthetic laryngoscope. AECOPD rat model was established by infusing bacteria at last. Results: in the model group, the rats were spiritually depressed, wheezing, breathing deep, airway secretion increased, pulmonary tissue volume increased, blood stasis appeared, the pathological results showed that the alveolar wall became thinner and larger, and the alveolar cavity fusion increased. Partial rupture and fusion formed alveoli, the number of alveoli decreased obviously, and inflammatory cells infiltrated around the alveoli, which coincided with the changes of human COPD. The pulmonary function of the model group was significantly lower than that of the normal control group (FEV0.3% FEV0.3% FVCV + FVC). Conclusion: the rat model of AECOPD can be successfully established by using rigid nasal endoscope, Panasonic camera monitoring system and self-made anesthetic laryngoscope, instilling elastase into the trachea and combining with bacterial attack.
【作者单位】：天津中医药大学第二附属医院;天津中医药大学;
【基金】：国家自然科学基金资助项目(81202796)
【分类号】：R-332;R563.9

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