大鼠抑郁模型的建立及其综合评价研究

发布时间：2018-06-22 19:29

本文选题：大鼠抑郁模型 + 悬尾次数　；参考：《亚太传统医药》2015年09期

【摘要】：目的:建立适宜于抗抑郁药物评价的抑郁大鼠动物模型及综合评价体系,为抗抑郁药物的药理药效评价奠定前期基础。方法:选用SD雄性大鼠,在CUMS法基础上,建立抑郁大鼠动物模型,考察造模前和造模7天、14天、21天、35天时大鼠体重、悬尾次数、糖水消耗、血清皮质酮等指标的变化,综合评价优选造模条件。结果:与空白组比较,造模21天模型组在体重、糖水消耗、悬尾次数、游泳时间、敞箱实验等方面均有明显降低趋势,大鼠血清皮质酮含量明显减少,实验结果证明了抗抑郁药物评价的抑郁大鼠动物模型建立的合理性。结论:该模型克服以往存在的工作量大,持续时间长、指标评价单一等问题,为抗抑郁药物的药效评价奠定实验基础。
[Abstract]:Aim: to establish an animal model and a comprehensive evaluation system for antidepressant drug evaluation in rats, so as to lay a foundation for the evaluation of pharmacological efficacy of antidepressant drugs. Methods: SD male rats were used to establish the depressive rat model on the basis of CUMS method. The changes of body weight, tail suspension times, sugar consumption and serum corticosterone were investigated before and after 7 days and 14 days and 21 days and 35 days after the establishment of the model. Comprehensive evaluation of optimal model making conditions. Results: compared with the blank group, the body weight, sugar water consumption, tail suspension times, swimming time, open box test and serum corticosterone content in the 21-day model group were significantly decreased. The results demonstrated the rationality of the animal model of antidepressant drug evaluation in depressive rats. Conclusion: this model overcomes the problems of heavy workload, long duration and single index evaluation, which can be used to evaluate the efficacy of antidepressants.
【作者单位】：辽宁中医药大学药学院;辽宁省组分中药工程技术研究中心;辽宁省现代中药研究工程实验室;
【基金】：国家自然科学基金(81241111) 辽宁省高等学校优秀人才支持计划资助项目(LR2013044)
【分类号】：R-332

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