刺五加注射液体外诱导大鼠骨髓基质细胞分化为神经元样细胞的研究

发布时间：2018-06-23 15:02

本文选题：骨髓基质细胞 + 刺五加注射液　；参考：《山东中医药大学》2008年硕士论文

【摘要】： 目的:1.探讨刺五加注射液体外诱导大鼠骨髓基质细胞(bone marrow stromal cells, BMSC)分化为神经元样细胞的最佳浓度及作用机制。2.以西药(β-巯基乙醇)做为对照组,探讨中药做为诱导剂的优势。方法:1.以不同浓度刺五加对BMSC进行体外诱导,用MTT法检测各组细胞生长增殖情况,用免疫细胞化学染色法计算各组神经元样细胞阳性数加以比较。2.以刺五加最佳药物浓度进行诱导,并以β-巯基乙醇(β-ME)做为实验对照组。通过形态学观察、免疫细胞化学染色法及尼氏体染色法计算各组神经元样细胞阳性数加以比较。结果:1.BMSC用不同浓度刺五加诱导后均可表现为典型的神经元样细胞形态,但以(0.3-0.4)mg/ml浓度诱导效果好,且对BMSC无明显细胞毒性。2.刺五加与β-ME两组细胞6h诱导率无明显差异,但随着时间延长,β-ME组阳性细胞数无明显增多,且死亡细胞数增多。刺五加组阳性细胞量随时间延长有所增多,且细胞死亡率远较β-ME少。结论:1.刺五加注射液体外诱导大鼠BMSC分化为神经元样细胞以(0.3-0.4)mg/mL浓度最佳。2.与西药诱导剂β-ME相比较,中药刺五加注射液毒副作用小,远期诱导效果好。
[Abstract]:Purpose 1. To investigate the optimal concentration and mechanism of Acanthopanax senticosus injection (Acanthopanax senticosus injection) in inducing the differentiation of rat bone marrow stromal cells (bone marrow stromal cells, BMSC) into neuron-like cells in vitro. Taking western medicine (尾-mercaptoethanol) as control group, the advantage of traditional Chinese medicine as inducer was discussed. Method 1: 1. BMSC was induced with different concentrations of Acanthopanax senticosus in vitro. MTT assay was used to detect the growth and proliferation of BMSC, and immunocytochemical staining was used to calculate the positive number of neuron-like cells in each group. The optimal concentration of Acanthopanax senticosus was used to induce and 尾 -mercaptoethanol (尾 -ME) was used as control group. Through morphological observation, immunocytochemical staining and Nissl body staining, the number of neuron-like cells in each group was calculated and compared. Results 1. BMSC induced by different concentrations of Acanthopanax senticosus showed typical neuron-like cell morphology, but the concentration of (0.3-0.4) mg/ml had a good induction effect and had no obvious cytotoxicity to BMSC. There was no significant difference in induction rate between Acanthopanax senticosus group and 尾 -ME group at 6 h, but with the prolongation of time, the number of positive cells and the number of dead cells in 尾 -ME group were not significantly increased. In Acanthopanax senticosus group, the number of positive cells increased with time, and the cell death rate was much lower than that of 尾-ME. Conclusion 1. In vitro, Acanthopanax senticosus injection induced rat BMSC to differentiate into neuron-like cells with the best concentration of (0.3-0.4) mg / mL. Compared with western medicine inducer 尾-ME, Acanthopanax senticosus injection has little side effect and good long-term induction effect.
【学位授予单位】：山东中医药大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R329;R285

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