hsa-miR-487b的靶基因预测及生物信息学分析

发布时间：2018-06-27 08:18

本文选题：miR + 生物信息学　；参考：《中华疾病控制杂志》2015年01期

【摘要】：目的对hsa-miR-487b进行靶基因的预测及功能分析,为进一步研究其功能提供线索。方法应用Pubmed和Google检索hsa-miR-487b相关文献,选择miRanda和Target Scan两种方法预测其靶基因,取其交集,合并已经实验证实的靶基因作为进一步生物信息学分析的基因集合,对此基因集合进行功能富集分析(GO分析)和Pathway分析。结果 hsa-miR-487b序列在各物种间高度保守;其预测靶基因富集于细胞增殖、转录调控、细胞内信号传导、膜转运、染色体的结构及修饰、基因表达调控、酪氨酸激酶活性等共16个生物学过程和功能上(P0.05),富集于Erb B信号通路、MAPK信号通路、Fc epsilon RI信号通路、癌症信号通路等7个通路,以及甲状腺癌、膀胱癌、子宫内膜癌、结直肠癌、急、慢性白血病这些疾病通路中(P0.10)。结论 hsa-miR-487b可能参与肿瘤发生及预后的生物学过程,为进一步实验性研究提供了线索。
[Abstract]:Objective to predict and analyze the target gene of hsa-miR-487b and provide clues for further study of its function. Methods the hsa-miR-487b literatures were searched by Pubmed and Google. Two methods, miRanda and Target scan, were selected to predict their target genes, and the intersection of them was selected. The target genes which had been confirmed by experiments were used as the gene sets for further bioinformatics analysis. Functional enrichment analysis (go analysis) and Pathway analysis were performed for this gene collection. Results the hsa-miR-487b sequence was highly conserved among different species, and its predictive target gene was enriched in cell proliferation, transcriptional regulation, intracellular signal transduction, membrane transport, chromosome structure and modification, gene expression regulation, and so on. Tyrosine kinase activity was found in 16 biological processes and functions (P0.05). It was enriched in 7 pathways, such as FC epsilon RI signaling pathway, cancer signal pathway, thyroid carcinoma, bladder cancer, endometrial carcinoma, colorectal cancer, and erb B signaling pathway. Acute, chronic leukemia in these disease pathways (P0.10). Conclusion hsa-miR-487b may be involved in the biological process of tumorigenesis and prognosis, which may provide clues for further experimental research.
【作者单位】：中国医科大学公共卫生学院流行病学教研室;沈阳医学院公共卫生学院卫生统计学教研室;
【基金】：国家自然科学基金(81272293)
【分类号】：R3416

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