经脾脏注射未成熟树突状细胞对大鼠小肠移植免疫耐受诱导作用的研究

发布时间：2018-06-27 14:38

本文选题：脾脏注射 + 树突状细胞　；参考：《南京医科大学》2009年硕士论文

【摘要】：目的:研究经脾脏注射骨髓来源的未成熟树突状细胞(iDC)对大鼠小肠移植免疫耐受的诱导。方法:分离供体F344大鼠骨髓来源DC细胞前体,经GM-CSF、IL-4体外刺激培养6天后,检测细胞表面共刺激分子及MHC表达,鉴定是否为imDC;健康成年F344大鼠为供体,SD大鼠为受体。随机分为四组(n=10),A组(阴性对照组):移植前7天,受体经阴茎背静脉注射生理盐水1ml;B组(阴性对照组):移植前7天,受体经脾脏注射生理盐水1ml;C组(iDC阴茎背静脉注射组):移植前7天,受体经阴茎背静脉注射供体的iDC,细胞数为2×106个;D组(iDC脾脏注射组):移植前7天,受体经脾脏注射供体的iDC,细胞数为2×106个。四组大鼠注射后1周后行大鼠异位节段性小肠移植术。观察:1、大鼠移植后存活情况;2、移植后7天移植肠病理学情况;3、同时TUNNEL法检测移植肠的细胞凋亡情况。结果: D组大鼠移植小肠存活时间为(17.50±1.05)d,较A组(7.17±1.17)d、B组(7.33±1.51)d、C组(12.83±2.79)d显著延长;D组移植小肠炎性细胞浸润、黏膜组织结构破坏程度明显低于A、B、C组;D组小肠粘膜上皮细胞凋亡数(9.3±1.7)个,相对于A组(25.3±4.6)个、B组(25.5±4.7)个、C组(16.5±2.1)个,移植肠粘膜上皮细胞凋亡数显著降低。结论:经脾脏注射供体来源的未成熟树突状细胞,可在一定程度上诱导受体产生免疫耐受,延长受体大鼠小肠移植术后的存活时间。D组移植小肠炎性细胞浸润、黏膜组织结构破坏程度和黏膜细胞的凋亡数目明显低于A、B、C组。
[Abstract]:Aim: to study the induction of immune tolerance of rat small bowel transplantation by immature dendritic cells (iDC) injected into spleen. Methods: DC cells derived from bone marrow of donor F344 rats were isolated and cultured in vitro for 6 days. The expression of costimulatory molecules and MHC on the surface of the cells was detected to determine whether it was imDC.The healthy adult F344 rats were used as donors and SD rats as the receptors. They were randomly divided into four groups: group A (negative control group): 7 days before transplantation, 1 ml of normal saline was injected into the dorsal vein of penis (negative control group): 7 days before transplantation, 1 ml of normal saline was injected into the dorsal vein of penis in group B (negative control group). The recipient was injected with 1 ml saline into the spleen (IDC group): 7 days before transplantation, the recipient was injected intravenously into the dorsal penile vein of the donor, the number of cells was 2 脳 106 D group (iDC splenic injection group): 7 days before transplantation, the recipient was injected into the dorsalis penile vein, and the number of cells was 2 脳 106 D group (iDC splenic injection group), 7 days before transplantation. The number of iDCs injected into spleen was 2 脳 106. Four groups of rats were treated with ectopic segmental small bowel transplantation 1 week after injection. The survival and pathology of grafted intestine were observed at 7 days after transplantation. The apoptosis of transplanted intestine was detected by Tunel method. Results: the survival time of small intestine transplantation in group D was (17.50 卤1.05) days, which was significantly longer than that in group A (7.17 卤1.17) d, group C (7.33 卤1.51) d (12.83 卤2.79) days, and the degree of mucosal structure damage was significantly lower than that in group A (9.3 卤1.7). Compared with group A (25.3 卤4.6), group B (25.5 卤4.7) and group C (16.5 卤2.1), the number of apoptotic cells decreased significantly. Conclusion: injection of donor derived immature dendritic cells into the spleen can induce immune tolerance to some extent and prolong the survival time of the recipient rats after small bowel transplantation. Group D can induce the infiltration of inflammatory cells in the transplanted small intestine. The degree of damage of mucosal structure and the number of apoptosis of mucosal cells were significantly lower than those of group A.
【学位授予单位】：南京医科大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R392

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