慢性HBV感染者外周血调节性T细胞水平及Foxp3与CD127的表达关系

发布时间：2018-06-27 16:23

本文选题：慢性HBV感染 + T淋巴细胞　；参考：《南昌大学》2010年硕士论文

【摘要】： 目的: 探讨慢性HBV感染者外周血中CD4+CD25+Foxp3+调节性T细胞(Regulatory T,Treg)的水平,并结合感染者的临床资料,分析Treg在HBV感染慢性化机制中的作用和与疾病进展的相关性,并初步研究其两种细胞标志—CD127分子与Foxp3转录因子表达之间的关系。方法: 采集34例免疫耐受期、26例免疫清除期和31例非活动或低(非)复制期慢性HBV感染者的外周全血,采用流式细胞染色技术,通过对CD4、CD25、Foxp3和CD127等细胞表面和内部标志进行荧光染色,用流式细胞分析仪设门圈定细胞群,检测CD4+CD25+Foxp3+ T细胞和CD4+CD25+CD127low T细胞的频率。结果: 1.在CD4+CD25+Foxp3+ T细胞占CD4+ T细胞的百分比中,免疫耐受组的比例(4.17±0.75)%高于非活动或低(非)复制组(3.66±0.85)%,差异具有统计学意义(Z=-2.693,P=0.007);且病毒阳性组(免疫耐受组+免疫清除组,HBeAg阳性,60例,(4.09±0.86)%)的比例高于病毒阴性组(非活动或低(非)复制组,HBeAg阴性,31例,(3.66±0.85)%)(t=2.266,P=0.026),差异具有统计学意义。将ALT异常组(免疫清除组)与ALT正常组(免疫耐受组+非活动或低(非)复制组,65例)的比例进行比较,结果显示两组的差异(3.99±1.00)%与(3.93±0.83)%之间无统计学意义(P0.05);再将免疫清除组26例感染者按ALT高低分为两个组(80 U/L,17例,(4.06±1.05)%和80 U/L,9例,(3.87±0.93)%),分析显示同样的结果,在两组中均无统计学意义(P0.05)。 2.在CD4+CD25+CD127low T细胞占CD4+ T细胞的百分比中,免疫耐受组(4.91±0.92)%和免疫清除组(4.94±1.34)%的比例均高于非活动或低(非)复制组(4.19±0.86)%,差异均具有统计学意义(t=3.251,P=0.002和t=2.558,P=0.013);且病毒阳性组(免疫耐受组+免疫清除组,HBeAg阳性,(4.93±1.11)% )的比例高于病毒阴性组(非活动或低(非)复制组,HBeAg阴性,(4.19±0.86)%)(t=3.208,P=0.002),差异具有统计学意义。将ALT异常组(免疫清除组)与ALT正常组(免疫耐受组+非活动或低(非)复制组)的比例进行比较,结果显示两组的差异(4.94±1.34)%与(4.57±0.95)%之间无统计学意义(P0.05);再将免疫清除组26例感染者按ALT高低分为两个组(80 U/L,17例,(4.93±1.48)%和80 U/L,9例,(4.98±1.11)%),分析显示同样的结果,在两组中均无统计学意义(P0.05)。3.将CD4+CD25+Foxp3+和CD4+CD25+CD127low检测的所有感染者的细胞频率进行比较,结果显示在两种表型检测的结果均值中前者的频率(3.95±0.88)%明显低于后者(4.68±1.08)%(Z=-4.718,P0.001),即用CD127low检测CD4+CD25+ T细胞的结果明显高于用Foxp3+检测的结果,两者之间的差异具有统计学意义。结论: CD4+CD25+调节性T细胞在慢性HBV感染者病毒高复制组中明显升高,并与感染者ALT的高低无明显关系。CD4+CD25+ T细胞中CD127分子的低表达与Foxp3转录因子的表达有一致性,但前者代表Treg的频率明显高于后者。
[Abstract]:Objective: to investigate the level of CD4 CD25 Foxp3 regulatory T cells in peripheral blood of patients with chronic HBV infection, and to analyze the role of Treg in the mechanism of chronic HBV infection and its correlation with the progression of HBV infection. The relationship between the expression of Foxp3 transcription factor and its two cell markers-CD127 was studied. Methods: peripheral whole blood samples were collected from 34 patients with chronic HBV infection in immune tolerance phase (n = 26) and chronic HBV infection (n = 31) in inactive or low (low) replication stage. Flow cytometry (FCM) was used. By fluorescent staining on the surface and internal markers of CD4, CD25, Foxp3 and CD127, the cell population was set up by flow cytometry, and the frequencies of CD4 CD25 Foxp3 T cells and CD4 CD25 CD127 low T cells were detected. Results: 1. In the percentage of CD4 CD25 Foxp3 T cells to CD4 T cells, The percentage of immune tolerance group (4.17 卤0.75)% was higher than that of inactive or low (non-replicating) group (3.66 卤0.85), the difference was statistically significant (ZAI-2.693P0. 007), and the proportion of virus positive group (immune tolerance group: 60 cases of HBeAg positive group, (4.09 卤0.86)%) was higher than that of virus negative group (non-live group). There were 31 cases (3.66 卤0.85) of HBeAg negative in motility or low (non-replicating) group (t = 2.266) (P < 0.026). The difference was statistically significant. The ratio of alt abnormal group (immune clearance group) and normal alt group (65 cases of inactive or low replication group) was compared. The results showed that there was no significant difference between the two groups (3.99 卤1.00)% and (3.93 卤0.83)% (P0.05), and 26 cases of infected persons in immune clearance group were divided into two groups according to alt level (80 UL / L 17 cases, (4.06 卤1.05)% and 80 UL / P 9 cases, (3.87 卤0.93)%). There was no statistical significance in both groups (P0.05). In the percentage of CD4 CD25 CD127low T cells to CD4 T cells, The percentage of immune tolerance group (4.91 卤0.92)% and immune clearance group (4.94 卤1.34)% was higher than that of inactive or low replication group (4.19 卤0.86), the difference was statistically significant (t _ (3.251) P _ (0.002) and t _ (2.558) P _ (0.013), and the proportion of virus positive group (immune tolerance group) was (4.93 卤1.11)%. HBeAg was significantly higher than that in viral negative group (inactive or low replication group, (4.19 卤0.86)%) (t = 3.208, P < 0.002). The difference was statistically significant. The ratio of alt abnormal group (immune clearance group) and normal alt group (immune tolerance group inactive or low (non-replicating) group) was compared. The results showed that there was no significant difference between the two groups (4.94 卤1.34)% and (4.57 卤0.95)% (P0.05), and 26 cases of infected persons in the immune clearance group were divided into two groups according to the alt level (80 UL / L 17 cases, (4.93 卤1.48)% and 80 U L / P 9 cases, (4.98 卤1.11)%). The cell frequencies of all infected individuals detected by CD4 CD25 Foxp3 and CD4 CD25 CD127low were compared. The results showed that the frequency of the former (3.95 卤0.88)% was significantly lower than that of the latter (4.68 卤1.08)% (ZP0.001), that is, the CD4 CD25T cell detected by CD127low was significantly higher than that by Foxp3, and the difference between them was statistically significant. Conclusion: CD4 CD25 regulatory T cells were significantly increased in chronic HBV infected patients with high replication. The low expression of CD127 in CD4 CD25 T cells was consistent with the expression of Foxp3 transcription factor, but the frequency of Treg in the former was significantly higher than that in the latter.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R392

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