龙岩市病毒性脑炎病例埃可病毒6型的基因特征

发布时间：2018-06-29 18:13

本文选题：埃可病毒型 + 病毒性脑炎　；参考：《海峡预防医学杂志》2015年06期

【摘要】：目的分析埃可病毒6型(ECHO6)龙岩市分离株的分子生物学特征。方法从2011年10株(7例儿童,3例成人)分离自脑脊液标本经血清中和鉴定的6株ECHO分离株中,选取3株(2例儿童,1例成人)进行VP1区核苷酸序列分析,并进行同源性比较及遗传进化分析。结果 3株ECHO6分离株VPl区序列长度为600个核苷酸,编码200个氨基酸;3株之间的核苷酸同源性为99%~100%,氨基酸同源性为96%~100%;与2010年福建龙岩分离株之间核苷酸同源性为94%,氨基酸同源性为95%~99%,基因分型一致,同属C2型。结论龙岩市2011年病毒性脑炎病例的ECHO6为C2型,成人感染病例值得关注。
[Abstract]:Objective to analyze the molecular biological characteristics of Echo virus 6 (ECHO6) strain in Longyan city. Methods from 10 strains of cerebrospinal fluid (CSF) isolated from 10 strains (7 children and 3 adults) in 2011 and identified by serum neutralization, 3 strains (2 children and 1 adult) were selected for VP1 region nucleotide sequence analysis. Homology comparison and genetic evolution analysis were carried out. Results the length of VPl region of three ECHO6 isolates was 600 nucleotides. The nucleotide homology and amino acid homology between the two hundred amino acid strains were 99100 and 960.The nucleotide homology and amino acid homology between the two isolates were 9410 and 95999 respectively, and the genotypes were identical and belonged to C2 type. Conclusion the ECHO6 of viral encephalitis in Longyan city in 2011 is type C 2, and the adult infection cases are worthy of attention.
【作者单位】：龙岩市疾病预防控制中心;福建医科大学龙岩教学基地;
【基金】：福建省青年科研课题(No.2012-1-47)
【分类号】：R373.2

【参考文献】