山东省1例脊髓灰质炎疫苗高变异株病例的变异分析

发布时间：2018-07-01 14:57

  本文选题：脊髓灰质炎病毒 + 非脊灰肠道病毒　； 参考：《病毒学报》2015年05期

【摘要】：为了解山东省2011年分离的1株脊髓灰质炎(脊灰)疫苗高变异株的基因特征,并对该病例接触者标本进行病原学鉴定及分析。本研究对采集的2份病例及40份接触者粪便标本进行病毒分离,对脊灰病毒进行全基因组序列测定和分析,对非脊灰肠道病毒进行VP1区序列测定,并将分离率较高的柯萨奇病毒(Coxsackievirus,CV)B1和CV-B3与国内外其他地区分离株进行VP1区同源性及系统进化树分析。其中病例标本中分离到1株脊灰病毒(P1/11186),1株CV-A4和1株CV-A8;接触者标本中分离到1株CV-A2、10株CV-B1、5株CV-B3和埃可病毒(Echovirus,E)3型、12型、14型各1株,提示当地可能存在肠道病毒流行,应加强监测。P1/11186为Ⅰ型脊灰疫苗病毒,在2A及3A区分别与Ⅱ型和Ⅲ型脊灰病毒发生重组,与相应的疫苗株相比全基因组共有25个核苷酸、9个氨基酸发生变异,其中VP1区8个核苷酸、5个氨基酸发生突变。CV-B1和CV-B3的同源性分析显示10株CVB1之间核苷酸同源性为97.0%~100%,氨基酸同源性为98.9%~100%;5株CV-B3之间核苷酸与氨基酸同源性分别为92.6%~100%与99.2%~100%。CV-B1与CV-B3VP1完整编码区进化树分析显示,山东分离株与国内其他省份分离株亲缘关系较近,位于同一基因簇,与世界其他地区分离株位于不同分支。
[Abstract]:In order to understand the gene characteristics of a poliomyelitis (polio) vaccine high variant strain isolated in Shandong Province in 2011, pathogenic identification and analysis were carried out on the samples of this case. In this study, the virus was isolated from 2 cases and 40 feces from contacts, the whole genome sequence of poliovirus was determined and analyzed, and the VP1 region sequence of non-polio enterovirus was sequenced. Coxsackievirus CV B1 and CV-B3 with high isolation rate were analyzed for VP1 region homology and phylogenetic tree analysis with other isolates at home and abroad. One strain of poliovirus (P1 / 11186), one strain of CV-A4 and one strain of CV-A8, one strain of CV-A2O10 strain CV-B1 / 5 strain CV-B3 and one strain of Echovirusus E (Echovirusus E) type 12, were isolated from the samples of the patients, indicating that there may be an epidemic of enterovirus in the area, and one strain of CV-A4 strain and one strain of CV-A8 strain were isolated from the specimen of the case. We should strengthen the surveillance. P1 / 11186 was a type I polio vaccine virus, which was recombined with poliovirus type 鈪，

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