利用鸡胚模型半体内评价多孔磷酸钙骨修复材料血管化的研究

发布时间：2018-03-15 08:57

本文选题：鸡胚绒毛尿囊膜　切入点：多孔磷酸钙骨修复材料　出处：《无机材料学报》2017年06期 　论文类型：期刊论文

【摘要】：利用鸡胚绒毛尿囊膜(CAM)中丰富的血管建立半体内模型,研究多孔磷酸钙骨修复材料的血管化。将多孔羟基磷灰石陶瓷(HA)和具有部分孔隙结构的磷酸钙骨水泥(CPCs)分别植入CAM中共培养,利用体式显微镜和扫描电子显微镜(SEM)观察CPCs表面血管的生长,并利用Image-Pro Plus和Nano Measurer定量血管密度、直径和数量;对CAM中培养的HA采用不脱钙组织学处理和观察。体式显微镜观察及定量结果显示多孔结构对血管生长影响显著,随着培养时间延长,多孔部分血管密度和数量均有明显增加,而无孔部分血管生长缓慢。CPCs表面血管以出芽方式生长,形成血管网络,直径一般小于50μm。SEM观察显示血管紧贴CPCs表面生长。苏木精-伊红(HE)染色显示血管通过孔隙结构长入HA内部。实验结果说明三维孔隙结构利于血管的生长和长入,此实验可简便、高效、低成本地研究多孔磷酸钙骨修复材料的血管化,为快速评价生物材料血管化寻找一种新途径。
[Abstract]:Using the abundant blood vessels in chorioallantoic membrane (CAM) of chorioallantoic chorioallantoic membrane, The vascularization of porous calcium phosphate bone repair materials was studied. The porous hydroxyapatite ceramic (HA) and the partially porous calcium phosphate cement (CPCs) were co-cultured in CAM respectively. The vascular growth on the surface of CPCs was observed by volume microscope and scanning electron microscope (SEM), and the density, diameter and quantity of blood vessels were measured by Image-Pro Plus and Nano Measurer. The HA cultured in CAM was treated and observed by undecalcified histology. The results of volume microscopy and quantitative analysis showed that the porous structure had a significant effect on the growth of blood vessels, and the density and number of the porous parts increased obviously with the increase of culture time. However, some of the vessels without pores grow slowly. The surface vessels of CPCs grow in the form of bud, forming a network of blood vessels. The observation of diameter less than 50 渭 m. SEM showed that the blood vessel grew close to the surface of CPCs. Hematoxylin-eosin) staining showed that the blood vessel grew into the HA through the pore structure. The experimental results showed that the three-dimensional pore structure was beneficial to the growth and entry of the blood vessel, and the experiment was simple and convenient. The vascularization of porous calcium phosphate bone repair materials was studied efficiently and cheaply in order to find a new way for rapid evaluation of vascularization of biomaterials.
【作者单位】：西南交通大学材料先进技术教育部重点实验室材料科学与工程学院;成都军区总医院口腔科;
【基金】：国家重点基础研究发展计划(973)项目(2012CB933602) 国家自然科学基金(51372210) 四川省应用基础(重点)项目(2016JY0011) 教育部博士点基金(20130184110023) 中央高校基本科研业务费专项基金(2682016YXZT11) 四川省高校科研创新团队建设计划项目(14TD0050)~~
【分类号】：R318.08

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