羧甲基壳聚糖温敏凝胶诱导骨髓间充质干细胞向神经细胞分化

发布时间：2018-03-18 15:26

本文选题：壳聚糖　切入点：骨髓　出处：《中国组织工程研究》2017年18期 　论文类型：期刊论文

【摘要】：背景:壳聚糖生物材料能够诱导骨髓间充质干细胞向神经细胞分化,壳聚糖衍生物羧甲基壳聚糖是否也能够诱导骨髓间充质干细胞向神经细胞分化尚不清楚。目的:探讨羧甲基壳聚糖温敏凝胶对骨髓间充质干细胞向神经细胞分化的影响及其可能的机制。方法:取传至3代的大鼠骨髓间充质干细胞用不同质量浓度(0,50,100,150,200,500 g/L)羧甲基壳聚糖温敏凝胶浸提液培养,对照组不添加羧甲基壳聚糖温敏凝胶浸提液。MTT法检测不同质量浓度羧甲基壳聚糖温敏凝胶浸提液对骨髓间充质干细胞增殖的影响,Western blot检测150 g/L羧甲基壳聚糖温敏凝胶浸提液对骨髓间充质干细胞NSE、Nestin、GAFP、Vimentin、NF-M、MAP-2、β3-tubulin以及Notch通路信号分子Notch1,Jag1表达水平的影响。结果与结论:(1)羧甲基壳聚糖温敏凝胶浸提液促进骨髓间充质干细胞增殖,在质量浓度为150 g/L时,增殖率达到最高水平;(2)150 g/L羧甲基壳聚糖温敏凝胶组NSE、Nestin、GAFP、Vimentin、NF-M、MAP-2、β3-tubulin的蛋白表达水平明显高于对照组,表明羧甲基壳聚糖温敏凝胶能够诱导骨髓间充质干细胞向神经细胞分化;(3)羧甲基壳聚糖温敏凝胶组Notch1和Jag1的蛋白表达水平明显低于对照组,表明其在诱导分化过程中能抑制Notch信号通路的活性。
[Abstract]:Background: chitosan biomaterials can induce bone marrow mesenchymal stem cells to differentiate into neural cells. It is not clear whether chitosan derivative carboxymethyl chitosan can also induce the differentiation of bone marrow mesenchymal stem cells into neural cells. Objective: to investigate the differentiation of bone marrow mesenchymal stem cells into neural cells by carboxymethyl chitosan thermosensitive gel. Methods: rat bone marrow mesenchymal stem cells (BMSCs) were cultured with different concentrations of different concentrations of 0 50g / 100g / L carboxymethyl chitosan thermosensitive gel. Effect of different concentration of carboxymethyl chitosan thermo-sensitive gel extract on proliferation of bone marrow mesenchymal stem cells in control group. The effects of gel extract on the expression levels of NSE-Nestin-GAFP- VimentininCon NF-MfMAP-2, 尾 _ 3-tubulin and Notch signaling molecule Notch1- Jag1. Results and conclusion the proliferation of bone marrow mesenchymal stem cells was promoted by the solution of the thermally sensitive gel extract of carboxymethyl chitosan, which was isolated from the bone marrow mesenchymal stem cells. When the mass concentration was 150 g / L, the protein expression level of NSE Nestinine GAFP Vimentinn (NF-MMAP-2), 尾 3-tubulin (尾 3-tubulin) group was significantly higher than that of the control group (P < 0.05), and the proliferation rate reached the highest level at a concentration of 150 g 路L ~ (-1) / L carboxymethyl chitosan temperature-sensitive gel group. The results showed that carboxymethyl chitosan thermosensitive gel could induce bone marrow mesenchymal stem cells to differentiate into neural cells. The protein expression levels of Notch1 and Jag1 in carboxymethyl chitosan temperature-sensitive gel group were significantly lower than those in control group. These results suggest that it can inhibit the activity of Notch signaling pathway during differentiation induction.
【作者单位】：河南大学淮河医院神经外科;郑州大学第一附属医院神经外科;
【分类号】：R318.08

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