医用多孔钽材料复合大鼠软骨细胞的生长特性及功能变化

发布时间：2018-04-11 06:56

  本文选题：多孔钽 + 生物相容性材料　； 参考：《解放军医学杂志》2014年06期

【摘要】：目的评价多孔钽材料复合大鼠软骨细胞的体外生长特性及功能变化。方法选取3周龄SD大鼠,分离关节软骨细胞并进行体外扩增培养,取生长状态良好的第2代细胞,鉴定后以1×106/ml密度接种于多孔钽上进行复合培养。采用倒置相差显微镜、扫描电镜、透射电镜观察软骨细胞在多孔钽上的形态特点,比色法测定复合培养的软骨细胞中糖胺多糖(GAG)的含量。结果经Ⅱ型胶原免疫细胞化学染色、甲苯胺蓝染色及番红O染色鉴定所获细胞为软骨细胞。倒置相差显微镜观察可见多孔钽边缘及培养板底部黏附大量软骨细胞;扫描电镜观察可见软骨细胞在多孔钽表面及孔隙内黏附、伸展、增殖,并分泌细胞外基质;透射电镜观察显示软骨细胞内超微结构大小及形态无明显改变;比色法检测显示软骨细胞在多孔钽支架上可持续分泌GAG。结论多孔钽材料与软骨细胞体外共培养显示出良好的生物相容性,适合作为软骨组织工程的支架材料。
[Abstract]:Objective to evaluate the growth characteristics and functional changes of porous tantalum composite chondrocytes in vitro.Methods the articular chondrocytes were isolated from 3-week-old SD rats and cultured in vitro. The second generation cells in good growth state were identified and cultured on porous tantalum with a density of 1 脳 106/ml.The morphologic characteristics of chondrocytes on porous tantalum were observed by inverted phase contrast microscope, scanning electron microscope and transmission electron microscope. The content of glycosaminoglycan (GAG) in chondrocytes was determined by colorimetry.Results the chondrocytes were identified as chondrocytes by immunocytochemical staining of type 鈪，

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