大鼠筋膜源性细胞与肌源性细胞成骨能力的对比研究

发布时间：2018-05-05 16:44

本文选题：肌细胞 + 细胞分化　；参考：《中国组织工程研究》2017年16期

【摘要】：背景:自体骨髓来源的间充质干细胞具有多分化潜能,能向成骨、成软骨细胞分化,加速骨组织及软骨组织的形成,但是成骨能力各不相同,哪种细胞的成骨能力更强有待进一步研究。目的:比较大鼠筋膜源性细胞与肌源性细胞的成骨能力。方法:从20只大鼠骨骼肌筋膜分离出筋膜源性细胞,从骨骼肌肌腹中分离出肌源性细胞。用流式细胞仪分选筋膜源性细胞和肌源性细胞,培养细胞。利用retro-BMP4病毒转染FDC-LacZ细胞。经过2次转染,转染的筋膜源性细胞通过Lac Z和碱性磷酸酶的染色而测定出转染效率。结果与结论:肌源性细胞产生的软骨细胞、钙盐沉积均多于筋膜源性细胞。提示大鼠肌源性细胞的成骨能力强于筋膜源性细胞。
[Abstract]:Background: mesenchymal stem cells derived from autologous bone marrow have the potential to differentiate into osteoblasts, chondroblasts, and accelerate the formation of bone tissue and cartilage tissue, but the osteogenic capacity is different. Which cell has more osteogenic ability needs further study. Objective: to compare the osteogenic ability of rat fascia derived cells and myogenic cells. Methods: fascial-derived cells were isolated from 20 rat skeletal muscle fascia and myogenic cells were isolated from skeletal muscle belly. The fascia derived cells and myogenic cells were separated by flow cytometry and cultured. FDC-LacZ cells were transfected with retro-BMP4 virus. After twice transfection, the transfected fascia derived cells were stained with Lac Z and alkaline phosphatase to determine the transfection efficiency. Results and conclusion: the calcium deposition of chondrocytes produced by myogenic cells was more than that of fascial-derived cells. The results suggest that the osteogenic ability of rat myogenic cells is stronger than that of fascial-derived cells.
【作者单位】：郑州大学第一附属医院骨科;
【基金】：国家自然科学基金资助项目(81472136)~~
【分类号】：R318.08

【参考文献】