有序聚甲基丙烯酸甲酯电纺纳米纤维作为大鼠背根神经元负载支架的可行性研究

发布时间：2018-05-21 11:27

本文选题：静电纺丝 + 聚甲基丙烯酸甲酯　；参考：《第三军医大学学报》2015年04期

【摘要】：目的探讨具备有序或无序拓扑结构的聚甲基丙烯酸甲酯(polymethylmethacrylate,PMMA)电纺纳米纤维材料作为原代大鼠背根神经元(dorsal root ganglion neurons,DRGn)负载支架的可行性。方法构建具有随机或有序拓扑结构的PMMA电纺纳米纤维;利用PMMA薄膜组作为对照,分离纯化大鼠原代DRGn,利用慢病毒技术转染绿色荧光蛋白基因作为显色手段,分析在随机及有序纤维支架上DRGn神经突的生长能力以及神经突和电纺纤维的依存关系。结果大鼠原代DRGn能够顺利在PMMA材料上贴壁并生长,在培养第2天,较之平面环境,DRGn未见平均神经突数量及神经突长度的明显区别,电纺纤维的拓扑结构对于DRGn神经突的生长具有明显的接触引导作用,在有序电纺纤维上DRGn能够生成和基质纤维延伸方向一致的神经突;通过GFP表达与背景纤维的合成图,发现在随机或有序电纺纤维上,DRGn神经突均能循纤维向前生长,但相比随机纤维,神经突似乎更倾向于接受有序电纺纤维的引导。结论有序PMMA电纺纳米纤维具有作为神经损伤后大鼠DRGn负载支架的潜力。
[Abstract]:Objective to investigate the feasibility of polymethylmethacrylate PMMA (PMMA) electrospun nanofiber material with ordered or disordered topological structure as the primary dorsal root neuron dorsal root ganglion neurons DRGN loading scaffold. Methods PMMA electrospun nanofibers with random or ordered topological structure were constructed, the primary rat DRGN was isolated and purified by using PMMA film group as control, and the green fluorescent protein gene was transfected by lentivirus technique as a means of color rendering. The growth ability of DRGn nerve process and the dependence of nerve process and electrospun fiber on random and ordered fiber scaffolds were analyzed. Results the primary rat DRGn was able to adhere to the wall and grow smoothly on the PMMA material. On the second day of culture, there was no significant difference in the average number of neurites and the length of the neurite compared with the plane environment. The topological structure of the electrospun fiber has obvious contact guiding effect on the growth of the DRGn neurite. On the ordered electrospun fiber, DRGn can produce the neurite which is consistent with the extension direction of the matrix fiber, and is expressed with the synthesis diagram of the background fiber through the GFP expression. It was found that DRGn neurites could grow forward on random or ordered electrospun fibers, but they seemed to be more inclined to accept the guidance of ordered electrospun fibers than random fibers. Conclusion ordered PMMA electrospun nanofibers have the potential as DRGn loaded scaffolds after nerve injury in rats.
【作者单位】：重庆医科大学附属第一医院神经外科;重庆中山医院药剂科;
【基金】：国家临床重点专科建设项目(财社[2011]170号) 国家自然科学基金(81100906) 重庆市科委前沿与应用基础研究计划(CSTC2014jcyj A10061)~~
【分类号】：R318.08

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