天然抗氧化剂在丝素蛋白材料中的稳定性研究

发布时间：2019-02-23 18:50

【摘要】：天然抗氧化剂因其能清除体内多余自由基、延缓细胞衰老、防止细胞癌变等特性而被广泛应用于生物医疗领域。但因其在应用和保存过程中易被氧化失活,限制了其优良性能的发挥。因此我们选取了丝素蛋白材料作为抗氧化剂的稳定体系,并初步探究稳定机理。本文通过调控丝素蛋白成膜的过程,以及采取90%甲醇后处理工艺,制备了不同二级结构的丝素蛋白膜。利用红外光谱和X射线衍射光谱,测定出了各种丝素蛋白膜的结构。并研究了它们的降解性能,三种膜具有不同的降解性能,Silk II结构能延缓丝素蛋白膜的降解。利用DPPH自由基清除法和高效液相色谱(HPLC)评价了丝素蛋白膜中三种抗氧化剂的抗氧化活性和结构稳定性,发现维生素C(VC)在Silk I结构的丝素蛋白膜中的稳定性更好,而姜黄素在Silk II结构的丝素蛋白膜中的稳定性更好,丝素蛋白对表没食子儿茶素没食子酸酯(EGCG)的稳定作用有待考证。最后我们研究了抗氧化剂与丝素蛋白膜和丝素蛋白溶液的相互作用,发现Silk II结构的丝素蛋白膜与姜黄素结合紧密,而溶解到PBS中的丝素蛋白与VC和EGCG结合紧密。结合稳定性实验,可以得出,溶液中的丝素蛋白能通过与VC和EGCG紧密结合而稳定VC和EGCG的活性和结构;Silk II结构的丝素蛋白能与姜黄素紧密结合而稳定姜黄素的活性和结构。综上所述,丝素蛋白材料能作为较好的抗氧化剂载体。通过调控丝素蛋白的结构,能使装载的抗氧化剂获得更优良的释放性能和稳定性能。与丝素蛋白材料的组合,使得抗氧化剂在生物医药领域的应用具有了更多的可能性。
[Abstract]:Natural antioxidants are widely used in biomedical fields because of their properties of scavenging excess free radicals, delaying cell senescence and preventing cell carcinogenesis. However, it is easy to be oxidized and deactivated in the process of application and preservation, which limits its excellent performance. Therefore, we selected silk fibroin as the stability system of antioxidants, and explored the stabilization mechanism. In this paper, silk fibroin membranes with different secondary structures were prepared by regulating the process of fibroin membrane formation and adopting 90% methanol post-treatment process. The structure of silk fibroin films was determined by IR and X-ray diffraction spectra. The degradation properties of three kinds of membranes were studied. The degradation of silk fibroin films was delayed by, Silk II structure with different degradability. The antioxidant activity and structural stability of three antioxidants in silk fibroin membrane were evaluated by DPPH free radical scavenging method and high performance liquid chromatography (HPLC). It was found that vitamin C (VC) had better stability in the silk fibroin membrane with Silk I structure. The stability of curcumin in the fibroin membrane with Silk II structure was better, and the effect of fibroin on the stability of epigallocatechin and catechin-gallate (EGCG) remained to be studied. Finally, we studied the interaction of antioxidants with silk fibroin membrane and silk fibroin solution. It was found that the silk fibroin membrane with Silk II structure binds closely to curcumin, while the silk fibroin protein dissolved into PBS binds closely to VC and EGCG. Combined with the stability experiment, it can be concluded that fibroin in the solution can stabilize the activity of VC and EGCG and the structure of; Silk II by binding closely with VC and EGCG, and the fibroin can bind closely with curcumin to stabilize the activity and structure of curcumin. In conclusion, silk fibroin material can be used as a better antioxidant carrier. By regulating the structure of silk fibroin, the release and stability of the loaded antioxidants can be improved. The combination of silk fibroin materials makes the application of antioxidants in biomedical field more possible.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R318.08

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