透明质酸改性PHBV组织工程纳米纤维支架的研究

发布时间：2019-06-12 07:11

【摘要】：组织工程支架材料表面性质对细胞的黏附起着重要作用,进而影响细胞的增殖、分化等一系列生长过程.本研究采用天然生物大分子透明质酸(hyaluronic acid,HA)对聚(3-羟基丁酸酯-co-3-羟基戊酸酯)(Poly(3-hydroxybutyrate-co-3-hydroxyvalerate),PHBV)进行表面修饰以提高材料的表面生物活性.首先通过静电纺丝法制备PHBV纳米纤维支架,利用1,6-己二胺胺解在PHBV表面引入自由胺基,并以此为反应活性位点在水溶性的碳化二亚胺/N-羟基琥珀酰亚胺缩合剂体系中将透明质酸固定接枝在PHBV表面.SEM结果表明,静电纺丝制备的PHBV纳米纤维支架表面平滑程度高,纤维直径分布较均匀,且没有串珠;FTIR证明1,6-己二胺改性及透明质酸接枝改性PHBV纳米纤维支架材料均成功实现;茚三酮法表明PHBV表面胺基密度随胺解时间增加而增大,在胺解约50min时达到最大值;水接触角法表明固定接枝透明质酸后,表面亲水性明显改善;细胞实验表明透明质酸改性的PHBV纳米纤维支架可显著促进软骨细胞的体外增殖.综上所述,透明质酸改性的PHBV纳米纤维支架可望应用于软骨组织工程领域.
[Abstract]:The surface properties of tissue engineering scaffolds play an important role in cell adhesion, and then affect a series of growth processes, such as cell proliferation, differentiation and so on. In this study, the surface modification of Poly (3-hydroxybutyrate-co-3- hydroxyvalerate) (Poly (3-hydroxybutyrate-co-3-hydroxyvalerate), PHBV) with natural biological macromolecule hyaluronic acid (hyaluronic acid,HA) was carried out to improve the surface biological activity of the material. Firstly, PHBV nanofiber scaffolds were prepared by electrospinning. Free amino groups were introduced on PHBV surface by 1, 6-hexanediamine hydrolysis, and hyaluronic acid was fixed and Graft onto PHBV surface in water-soluble carbodiimide / N-hydroxysuccinimide condensation agent system. The results showed that the surface of PHBV nanofiber scaffolds prepared by electrospinning had high surface smoothness and uniform fiber diameter distribution. And there are no beads; FTIR showed that 6-hexanediamine modified and hyaluronic acid grafting modified PHBV nanofiber scaffolds were successfully realized. Ninhydrin method showed that the surface amino density of PHBV increased with the increase of amine hydrolysis time and reached the maximum when amination was about 50min. Water contact angle method showed that the surface hydrophilicity of PHBV nanofiber scaffolds modified by hyaluronic acid was significantly improved after fixed grafting hyaluronic acid. Cell experiments showed that hyaluronic acid modified PHBV nanofiber scaffolds could significantly promote the proliferation of cartilage in vitro. In conclusion, hyaluronic acid modified PHBV nanofiber scaffolds are expected to be used in cartilage tissue engineering.
【作者单位】：湖南大学材料科学与工程学院;喷射沉积技术及应用湖南省重点实验室;湖南大学生物学院;湖南省中医药研究院;
【基金】：教育部新世纪优秀人才(NECT-12-0170) 湖南省自然科学基金(2016JJ2023) 湖南省研究生创新基地(2014-2017) 湖南大学交叉学科研究资助项目(2014JCA09)~~
【分类号】：R318.08

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