负载不同官能团的PBT平板膜对血浆蛋白吸附及血小板、白细胞黏附的初步研究

发布时间：2019-06-17 08:09

【摘要】：目的制备具有不同官能团(-CH3、-COOH、-OH、两性基团)的聚对苯二甲酸丁二醇酯(PBT)平板膜,并了解其对血浆蛋白吸附情况与对白细胞、血小板的黏附效果。方法利用紫外改性的方法,将不同单体接枝到PBT表面,得到PBT-AA(-COOH)、PBT-HEMA(-OH)、PBT-SMDB(两性基团)并进行表征。采用ELISA测定吸附的人血清白蛋白(HSA)、纤维蛋白原(Fgn)含量,扫描电镜(SEM)观察黏附白细胞、血小板数量。结果 HSA吸附量(μg/cm2),PBT、PBT-AA分别为:15.05±4.51vs 35.61±6.91(P0.01);Fgn吸附量(μg/cm2),PBT、PBT-HEMA、PBT-SMDB分别为:19.85±1.93 vs 14.93±4.05 vs 17.04±2.10(P0.05)。4种材料在吸附HSA、Fgn前后,黏附的WBC、Plt均发生明显变化(P0.01)。结论 Fgn对2种血小板和白细胞的黏附均有促进作用,而HSA对白细胞的黏附有一定抑制性。通过四种材料的比较发现,PBT-AA滤除白细胞及保留血小板的效果更好。
[Abstract]:Objective to prepare polybutylene terephthalate (PBT) plate membrane with different functional groups (- CH3,-COOH,-OH, amphoteric group) and to understand the adsorption of plasma protein and the adhesion effect of polybutylene terephthalate to leukocytes and platelets. Methods PBT-AA (- COOH), PBT-HEMA (- OH), PBT-SMDB) was obtained and characterized by grafting different monomers onto the surface of PBT by UV modification. The content of human serum albumin (HSA), fibrin (Fgn) was measured by ELISA, and the number of adherent leukocytes and platelets were observed by scanning electron microscope (SEM). Results the adsorption capacity of HSA (渭 g/cm2), PBT,PBT-AA was 15.05 卤4.51vs 35.61 卤6.91 (P 0.01); Fgn adsorption capacity (渭 g/cm2), PBT,PBT-HEMA,PBT-SMDB was 19.85 卤1.93 vs 14.93 卤4.05 vs 17.04 卤2.10). The adhesion WBC,Plt of the four materials changed significantly before and after HSA,Fgn adsorption (P 0.01). Conclusion Fgn can promote the adhesion of platelets and leukocytes, while HSA has a certain inhibitory effect on leukocyte adhesion. Through the comparison of the four materials, it was found that PBT-AA had better effect on leukocyte filtration and platelet retention.
【作者单位】：中国医学科学院北京协和医学院输血研究所;
【基金】：四川省科技支撑计划项目(项目编号:2008SZ0101) 成都市科技计划项目(11GGYB892SW-070)
【分类号】：R318.08;R446.112

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