异氟醚对人类胚胎干细胞自我更新能力及其分化的影响

发布时间：2017-12-31 05:22

本文关键词：异氟醚对人类胚胎干细胞自我更新能力及其分化的影响　出处：《青岛大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的:探讨异氟醚对人类胚胎干细胞自我更新能力及分化情况的影响。方法:将传代后1天的细胞随机分为实验组和对照组,每组n=5。实验组细胞暴露于2%异氟醚、5%CO2和21%O2的混合气体6h;对照组细胞暴露于5%CO2和21%O2的混合气体6h。观察两组胚胎干细胞的生长情况,对两组细胞计数并检测两组细胞干性基因及各胚层标志性基因的m RNA水平。结果:传代后第五天,两组细胞集落出现不同,实验组细胞数目明显低于对照组,差异有显著性(t=3.40,P0.01);实验组多能性基因(Nanog,Sox2,Oct4,Lin28)对应的m RNA水平低于对照组,差异有显著性(t=2.82~3.89,P0.05);实验组内胚层、中胚层、外胚层标志性基因(AFP,T,Nestin)对应的m RNA水平均高于对照组,差异有显著性(t=2.67~3.33,P0.05)。结论:异氟醚能够抑制离体人类胚胎干细胞的自我更新能力,并促进其提前分化。
[Abstract]:Objective: To investigate the effect of isoflurane on the self-renewal and differentiation of human embryonic stem cells. Methods: after 1 days the cells were randomly divided into experimental group and control group, each group of experimental group n=5. cells were exposed to 2% isoflurane, 6h 5%CO2 and 21%O2 mixed gas mixed gas; control group 6h. cells exposed to 5%CO2 the two groups were observed and 21%O2 embryonic stem cell growth, cell counting and detection of two groups of two groups of stemness genes and the gene m germ layer marker RNA level. Results: fifth days after passage, two groups of cell colonies appeared different, the number of cells in experimental group was significantly lower than the control group, there was significant difference (t=3.40, P0.01); the experimental group of pluripotency genes (Nanog, Sox2, Oct4, Lin28) corresponding to the m RNA level lower than the control group, there was significant difference (t=2.82~3.89, P0.05); experimental group of endoderm, mesoderm and ectoderm marker genes (AFP, T, Nestin) The corresponding level of M RNA was higher than that of the control group, the difference was significant (t=2.67~3.33, P0.05). Conclusion: Isoflurane can inhibit the self-renewal ability of human embryonic stem cells and promote its early differentiation.

【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R614

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