补阳还五汤配合甲强龙缓释凝胶预防腰椎手术失败综合征

发布时间：2018-01-17 01:12

本文关键词：补阳还五汤配合甲强龙缓释凝胶预防腰椎手术失败综合征　出处：《河南中医药大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的:1.明确甲强龙玻璃酸钠缓释凝胶对腰椎手术失败综合征的预防作用;2.明确补阳还五汤在预防腰椎腰椎手术失败综合征过程中与甲强龙玻璃酸钠缓释凝胶的协同作用。方法:将30只雄性白兔随机分为A(n=10),B(n=10),C(n=10)三组,适应性喂养一周后,手术术切开背部,咬除T9棘突及椎板,暴露脊髓。以动脉夹钳夹脊髓15s造模。造模后A组常规缝合,B,C组术区填充3ml玻璃酸钠甲强龙缓释凝胶后缝合。术后第二日开始,每日对C组白兔行补阳还五汤灌胃处理,每日给药剂量为6.5ml/kg。术后当天,1周,3周,6周分别对白兔进行BBB评分检测及神经电生理检测。术后第6周,以T9段为中心采集脊柱标本制作组织切片,进行HE染色及免疫荧光染色。观察记录各检测指标,收集整理数据,进行统计分析。结果:行为能力检测结果显示:A、B、C三组在造模后的功能恢复的速度和程度呈依次递增趋势。C组功能恢复速度较快,程度较高,B组次之,A组则最差。神经电生理检测结果显示:术后所有时间点sep潜伏期与波幅与手术当天比较均有统计学意义(p0.05),C组SEP潜伏期曲线较其他两组低,且下降更为迅速。C组SEP波幅则较其他两组高,且上升更快。HE染色结果显示:A,B,C三组HE染色瘢痕面积依次递减,各组间差异有统计学意义(p0.05);免疫荧光染色结果显示:A,B,C三组免疫荧光半定量数值依次递减,各组间差异有统计学意义(p0.05)。结论:1.甲强龙玻璃酸钠缓释凝胶能预防腰椎手术失败综合征的发生;2.补阳还五汤配合甲强龙缓释凝胶能够增强对腰椎手术失败综合征的预防作用。
[Abstract]:Objective 1. To determine the preventive effect of sodium methylhyaluronate sustained-release gel on lumbar failure syndrome. 2. To clarify the synergistic effect of Buyang Huanwu decoction and sodium methylhyaluronate sustained-release gel in the prevention of lumbar vertebrae failure syndrome. Methods: thirty male white rabbits were randomly divided into two groups. After one week of adaptive feeding, the back was cut off and the T9 spinous process and lamina were removed. The spinal cord was exposed. The spinal cord was clamped with artery clamp for 15 s. The group A was sutured with 3ml sodium hyaluronate methanolone sustained-release gel and then sutured. The operation area of group A was sutured with 3ml sodium hyaluronate methylketene sustained-release gel. The operation began on 2nd after operation. Group C rabbits were treated by oral administration of Buyang Huanwu decoction daily, the daily dose was 6.5 ml / kg, 1 week after operation and 3 weeks after operation. At 6 weeks, the BBB score and neuroelectrophysiology of rabbits were detected respectively. At the 6th week after operation, the spinal specimens were collected at the center of T9 segment to make tissue sections. He staining and immunofluorescence staining were performed. The detection indexes were observed and recorded, and the data were collected and collected for statistical analysis. The recovery speed and degree of functional recovery in group C showed an increasing trend in turn. The speed of functional recovery in group C was faster than that in group B, followed by group B. The results of electrophysiologic examination showed that the latency and amplitude of sep at all time points after operation were significantly higher than those on the day of operation (P 0.05). The latency curve of SEP in group C was lower than that in the other two groups, and decreased more rapidly. The amplitude of SEP in group C was higher than that in the other two groups. The scar area of HE staining in C group was decreased in turn, and the difference between each group was statistically significant (P 0.05). The results of immunofluorescence staining showed that the semi-quantitative value of immunofluorescence in group C decreased in turn. Conclusion: (1) Sodium methylhyaluronate sustained-release gel can prevent the occurrence of failed lumbar surgery syndrome. 2. Buyang Huanwu decoction combined with Jiqianglong sustained-release gel can enhance the preventive effect on failed lumbar surgery syndrome.
【学位授予单位】：河南中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R687.3

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