骨巨细胞瘤血清学分析和蛋白组学研究及临床意义

发布时间：2018-01-20 05:50

  本文关键词： 骨巨细胞瘤 血清Ⅰ型胶原交联氨基末端肽 抗酒石酸酸性磷酸酶5b iTRAQ 热休克蛋白　出处：《新疆医科大学》2015年博士论文　论文类型：学位论文

【摘要】：目的:探讨血清Ⅰ型胶原交联氨基末端肽(NTx)和抗酒石酸酸性磷酸酶5b(TRACP5b)在骨巨细胞瘤患者中表达及其临床诊断价值;探索热休克蛋白在骨巨细胞瘤中的潜在作用。方法:收集2011年6月至2014年12月就诊于本院且符合入组标准的GCT患者37例,其中男性20例,女性17例,年龄范围20~56岁,中位年龄31.6岁。采用酶联免疫吸附法(ELISA法)检测患者血清NTx和TRACP5b表达水平,并对其结果进行分析;同时提取4例患者骨巨细胞瘤瘤组织和肿瘤界限外5cm处正常骨组织对照,进行差异蛋白检测。结果:11例骨巨细胞瘤5cm患者和26例骨巨细胞瘤≥5cm患者的血清NTx,TRACP5b比较,骨巨细胞瘤≥5cm的患者血清NTx,TRACP5b含量均升高,差异有统计学意义(P0.05)。与X线分级I期骨巨细胞瘤患者NTx,TRACP5b含量比较,II期NTx,TRACP5b含量无升高,差异无统计学意义(P0.05);III期显著升高,差异有统计学意义(P0.05)。与组织学I期骨巨细胞瘤患者NTx,TRACP5b含量比较,II期NTx,TRACP5b含量无升高,差异无统计学意义(P0.05);III期显著升高,差异有统计学意义(P0.05)。肿瘤位置对血清NTx水平和TRACP5b有显著影响(P0.05);iTRAQ技术分析骨巨细胞瘤相关的差异表达蛋白,筛选出3个热休克蛋白:HS90A,HSPB1,HSPB2。结论:血清NTx和TRACP5b是反映GCT骨代谢的一个灵敏而简便的生化指标,对骨肿瘤诊断及鉴别诊断有一定的临床意义,两者联合检测更有利于骨巨细胞瘤患者临床病理特征分级的预测;骨巨细胞瘤中的iTRAQ分析是一个蛋白组学检查的有效方法,本研究旨在鉴定出差异表达蛋白,即热休克蛋白,该蛋白是骨巨细胞瘤侵袭性行为的重要预测物。
[Abstract]:Objective: to investigate the expression and clinical diagnostic value of serum type I collagen cross-linked amino-terminal peptide (NTX) and tartrate-resistant acid phosphatase (5bCP-5b) in patients with giant cell tumor of bone (GCT). To explore the potential role of heat shock protein (HSPs) in giant cell tumor of bone. Methods: from June 2011 to December 2014, 37 GCT patients who met the criteria of admission were collected. Among them, 20 cases were males and 17 cases were females. The age range was 20 ~ 56 years old. The median age was 31.6 years old. The expression of NTx and TRACP5b was detected by Elisa and the results were analyzed. At the same time, 4 patients with giant cell tumor of bone tissue and 5 cm beyond the tumor boundary of the normal bone tissue control. Results the serum NTxCT-TRACP5b levels were compared in 11 patients with giant cell tumor of bone (5 cm) and 26 patients with giant cell tumor of bone (鈮，

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